Studies On Gene Location And Fertility Restoration Performance Of Male Sterile Lines Of Wheat With Ae.bicornis Cytoplasm

Four male sterile lines of different nuclei genotypes with the same cytoplasm of Aeglops bicornis ,ms(bicorj-83 (37) 65. ms(bicor)-yanshi 9^ ms(bicor)-80 (6) and ms(bicor)-90-110, were selected as the materials in the study on gene location of B -type cytoplasmic male sterile lines by using monisomic analysis and nullisomic -tetrasomic analysis methods. It revealed that there was a major restoring fertility gene against AeMcornis cytoplasm on the chromosome IB of Chris. Other chromosomes except IB of Chris have no major fertility-restoring gene. But it can't eliminate the possibility of genes which can exhance the fertility and the inhibiting genes which reduce the fertility except IB of Chris.Five male sterile lines of wheat with AeMcornis cytoplasm , namely ms(bicor)-8222> ms(bicor>83 (37) 65 > ms(bicor)-yanshi 9^ ms(bicor>80 (6) and ms(bicor)-90-110, with some wheat varieties or lines and partial parent materials was investigated. The result were as follows: Y) In the five male sterile lines, with different nucleus, of wheat with AeMcornis cytoplasm there was obvious difference of restoring degree between the non-lB/lR wheat male sterile lines ms(bicory)-90-110 and the 1BL/1RS wheat male sterile lines ms(bicorj-83 (37)65 > ms(bicorj-Yanshi 9, while there was no obvious difference between the four 1B/1R male sterile lines each other; 2) As the same male sterile line tested crossing with different restoration lines, the restoration degree showed successive variation? 3) The restoration degree of non 1B/1R male sterile lines were higher than that of 1B/1R male sterile lines; 4) As the same restoration lines, there was obvious difference between each hybrids(Fi) derived from tested crossing.During the studies on gene location and fertility restoration performance, therelation of frequency of haploid and fertility was also studied. The result showed that the frequency of the FI hybrid of B -type was related to the difference of 1BL/1RS translocation chromosome of male sterile lines, and was related to the construction of chromosome. The frequency of haploid could be increased by the aneuploid. There was no relation between the frequency of haploid and fertility.Investigation on the transmission of gamete of B-type cytoplasm male sterile FI hybrid showed that both the male and female 1BL/1RS gamete transmission rate were less 50%, and 1BL/1RS gamete transmission was lower than IB gamete transmission. It was obvious that the male gamete 1BL/1RS transmission was even lower. The reason was that the interaction of 1BL/1RS translocation chromosome and alien cytoplasm.