Mapping With Marks And Effect Analysis Of The Fertility Restorer Genes For Male Sterile Line With Ae.kotschyi Cytoplasm In Wheat

Wheat male sterile line with Ae.kotschyi cytoplasm has the best potential application and was controlled by two pairs of major fertility restorer genes;The two pairs of major fertility restorer genes were located in Rfk1 for 1BS and Rfk2 for 2BL of wheat;Good restoring lines would be selected and hybrid wheat varieties would be developed by mapping restorer genes and analysising the effect of two restorer genes;In this study, the near-isogenic lines (NILs) were constructed using sterile line Yumai 3 with Ae.kotschyi cytoplasm which don't induced haploid, restorer line Yumai 2 which have two pairs of major restorer genes of Rfk1å'ŒRfk2;The F5 group of Yumai3/Yumai2 NILs and 5 sterile lines of Yumai3,Yunong43,Yujiao1,Yunong93151 and Yumai21;That materials wered be Markered and effect analysised,the results showed as follows:1,By using the SSR makers linkaged to restorer genes,which mapped and identified genes of Rfk1 and Rfk2 in F5 groups of Yumai3/Yumai2 NILs;5 SSR makers of TAGLGAP,Xgwm374,Xgwm18,Xgwm11,and BARC061wered identified in 1BS,which markers to Rfk1 distance are 10.1 cM,7.1 cM,3.0 cM,3.0 cM and13.4 cM, respectively. The Rfk1 mapped between Xgwm18 and Xgwm11;The genetic distance between Rfk2 and primer cfd267 was 5.2,Rfk2 and primer Xgwm526 was 18.8 cM.2. By identified genotypes in F5 groups of Yumai3/Yumai2 NILs and restoration performance in F1 groups of NILS/ Yumai3,which judged genotypes of F1 groups of NILS/ Yumai3;There are 69 plants carried Rfk1 restoring genes , which average restoring degrees was 64.92% with domestic method and 106.68% with international method; There are 56 plants carried Rfk2 restoring genes, which average restoring degrees is 57.82% with domestic method , 93.35% with international method;There are 66 plants carried two restoring genes, which average restoring degrees is 73.98% with domestic method and 117.37% with international method.Therefore, the restoring ability of Rfk1 is stroger than Rfk2 ,the 2 restoring genes have accumulative effect. At the same time , the low restoring ability (<45%) plants always carry Rfk2 gene.3, The hybridized combination NILs of 5 sterile lines with Ae.kotschyi cytoplasm Yunong43,Yujiao1,Yunong93151 and Yumai21 wered used in identifying the effect of Rfk1 and Rfk2 ;The result show the average restoring degrees of Rfk1 on 5 sterile lines was higher than Rfk2,Hence,the effect of Rfk1 is bigger than Rfk2.