Study On Roles Of Dietary Mannan Oligosaccharide In Sanhuang Broilers

It has become clear over the last decade that the group of non-digestib1e oligosaccharides(NDO) pla}r, or are like1y to pla}', a variety of physio1ogica1 and nutritiona1 roles inimproving gut microflora, enhancing body immunity, regulating endocrine secretion andincreasing nutrient avai1ability potentiall}', which prove to broaden its traditional function.NDO, a non--nutritional regulatof, has extensive prospect among dietary antibioticreplacements. This study U'as conducted to evaluate the effects of dietary mannanoligosaccharide (MOS ) on grox'1h performance, gut microflora, irnmune function andendocrine secretion in Sanhuang broilers of 0~4 weeks o1d, and to assess the function ofdietary MOS comprehensiveIy. The purpose of this study was to seri'e as the base for furtherresearch and application of oilgosaccharides in Sanhuang broi]ers.96 Sanhuang broilers of l-da3' old were randomly assigned to four groups, u'ith fOurreplications per group. The control was provided with basal diet. 0.075%MOS, 0.l%MOS.75bog/kgCTC were suppIemented to the basal diet respectively to fOrm trial diets. The birdswere fed their respective diets from l-day to 28 days old. At day 28, four broilers per group'vere slaughtered to collect samples fOr further assess. Peripheral blood was heparinized andassayed for T lymphoc}tes blastogenic responses to ConA and Macrophage activity. Serumwas separated and assessed fOr Hl antibody titres to Newcastle disease virus, and T3, T4, GHand lGF-1. Thymus of the 1eft part, Spleen and Bursa. F. were co1Iected for relative weight ofimmune organs(mg/g) of Sanhuang broilers. Fresh cecal contents were col1ected and analyze4for Bifidobacterium and Escherichia coli concentration.Results showed that at l~2l days of age, dietary MOS did not improve Daily Weight Gain(DWG ) and Feed Conversion Ratio (FCR) of Sanhuang broilers. DWG of broilers withi75mg/kgCTC increased by 4.30% and 5.3l% at 2, 3 weeks oId. 0.075% MOS increase4DWG and FCR of broilers of 2l~28 days old significant1y (Prt0.05 ), respectiveIy. There wasno significant difference on the groups supplemented with 0.l%MOS and 75mg/kg CTC-Dietary supplementation with 0.l%MOS and with 0.075% increase serum HI antibody titresto Newcast1e disease virus (P<0.05 ), T lymphocytes blastogenic responses to Con-A.Macrophage Activity (Prt0.05). Besides, MOS increased significantly the amount of1ymphoc}tes in Gut-ssociated Lymphoid Tissue (GALT ), which secreted SIgA.The concentration of Bifidobacteria in ceca was significantly higher and that of E. coli waslower in groups supplemented with 0. l%MOS and with 0.075% than that of control. Besides,it w'as shown that MOS improved cecal morPhology of Sanhuang broilers. Cry' depth wasconsistently reduced and the villus length increased in the MOS fed broilers.ln comparison to the ControI, MOS increased serum T3 and IGF-I significantly There is nosignificant difference in serum T4 and GH.