Studies On Biopharmaceutics Of Ultra-fine Powder Of Huanglian Jiedutang

OBJECTIVE : Ultramicro-pulverization technology is a high-tech that was developed rapidly in recent years. It has been gradually adopted in the processing of Chinese materia medica and appeared its special advantage. In order to offer scientific testify for the application of ultramicro-pulverization in the preparation of Chinese materia medica, this research investigated the effects of ultramicro-pulverization on Huanglian jiedutang' histological configurations, dissolving characteristics and bacteriostasis effects.METHODS: The research on the ultra-fine powder and conventional fine powder of Huanglian jiedutang and Huanglian, Huangbo,Huangqin,Zhizi as fellow: 1. To compare the histological configuration of ultra-fine powder and conventional fine powder with SEM and LM; 2. The qualitative test with TLC and the quantitative test with HPLC on the ultra-fine powder and conventional fine powder. 3. The pilot study on pharmacodynamics of HLJDT' ultra-fine powder with Bacteriostasis in vitro.RESULTS: 1. SEM and LM results show that the histological pieces were vivid and erose in the conventional fine powder; but in the ultra-fine powder, the particles were more uniform and improved in the spherical degree, the granularity became smaller. HLJDT conventional fine powder can also observe the histological configuration of Huanglian, Huangbo,Huangqin and Zhizi; in the HLJDT ultra-fine powder, it can't be observed, but improved in the mixed uniformity. Furthermore, the cell wall characteristics of conventional fine powder and ultra-fine powder was remarkably different in the same magnify multiple ( x 750) . The former can observe large quantities of cell flock which cell wall was integrate, the latter was on the contrary. 2. The qualitative test results show that the TLC of HLJDT ultra-finepowder has obvious changes, but the simply extract can't effect the TLC. The quantitative test results show that the ultra-fine powder has a higher content of effective component than the conventional fine powder, improved 34.63% in Huangqnu 50.96% in Zhizi, 37.07%, 32.53%, 26.66% in HLJDT' Berber, Baicalin, Geniposide by ultrasonic extract, and 51.15%, 45.50% in HLJDT' Baicalin, Geniposide by simply extract(stirred by spoil water). The HPLC results show that two kinds of powder have the similar absorb peak in the same reserve time, but peak magnitude is different. 3. The Bacteriostasis in vitro results show that two kinds of powder' Bacteriostasis circle wasn't obvious difference in the conventional extract, but existed remarkably difference in simply extract (stirred by spoil water)(p<0.01).CONCLUSION: The ultra-fine powder particles were more uniform in shape and became smaller in size, the mixed uniformity of HLJDT improved remarkably; Nearly all plant cells were cracked in SEM; the dissolving characteristics changed, the extract processing can be simplified; the dissolving content increased and bacteriostasis effect improved.