Study Of Abnormality And Mutation Of Experimental Chronic Fluorosis In Chicks

To understand the effect of chronic fluorosis on abnormality and mutation in chicks,36 one-day LuoManHe cocks were randomly divided into 6 groups of 6 each. I group drinking running water was control group, II ~ VI groups were fed with the fluorine water of 1.75g/L(l/27LD50),0.875 g/L,0.438g/L,0.219g/L,0.109g/L,respectively,for 90d.The Content of RBC and Hb, micronucleus rate and chromosome damage were observed. At the same time, 10 three-month cocks were randomly divided into 2 groups of 5 each.Test group drinked water with 3.25g/L sodium fluoride and control one with running water,for 120 days.Sperms was collected at the time of 60,80,100,120 day ,respectively,abnormality rate,energy rate and density of sperms were determined.After 120 days,spermaries were weighed , microkernel rate of sperm cell and pathologic changes of visceral organs were observed.The results were as follow:(1) Compared with the control group.the content of RBC and Hb in all fluorosed groups were decreased(P<0.01),moreover,showed a significant dose-effect correlation;(2)The Cell rate of chromosome aberration of II ~V groups was higher than control group and difference was obvious(P<0.01),It showed the obvious correlativity with the quantity of fluorine in the water; (3)the micronucleus rate of II ~IV group was higher than control group and difference was obvious(P<0.01),It showed the obvious correlativity with the quantity of fluorine in the water; (4) High fluorine injured greatly tissue structure of visceral organs especially liver and kidney; (5) Size and weight of spermaries,sperm amount and energy rate were lower man control group.sperm abnormality rate and microkernel rate were higher,they showed the obvious correlativity with time,the difference was obvious(P<0.01). Chronic fluorosis in chicks can damage chromosome ,visceral organs and sperms.High fluorine was the mutagenic and malformed material.