The Research On The Relationship Between Sutai Pig Pork Quality Traits And The Microsatellites Of Chromosome 15

100 SuTai fattening pigs were slaughtered and their carcass weight, BFT (backfat thickness), tenderness, marbling, WHC (water-holding capacity), pH value and IMF (intramuscular fat content ) were measured. The result of descriptive statistics shows that the BFT of Sutai pig is very thin (2.58 0.57cm) and the marbling score is favorable(3.24 0.57) and the pH value (6.09 0.1 9) of muscle coincide with that reported. But it is notable that the tenderness and the IMF, important to pork quality, vary greatly. According to the analysis by SPSS, the correlation between different traits is significant or extremely significant between carcass weight and BFT, marbling, pH value, tenderness (r: 0.569**, 0.223*, -0.253*, -0.233*); BFT and tenderness, IMF, marbling (r:-0.342**, 0.319**, 0.255*); marbling and tenderness, IMF(r: -0.26* , -0.51*); tenderness and pH value, IMF(r:-0.213*, -0.25*).Six microsatellites on chromosome 15 were selected to investigate polymorphism in Sutai pig population by PCR and PAGE electrophoresis. The result shows that 6 microsatellites are polymorphic and the fragments of PCR vary from 92bp to 302bp. The allele number varies from 3 to 7, and the frequency of allele from 0.01 to 0.57, heterozygosity from 0.52 to 0.79, polymorphism information content (PIC) from 0.42 to 0.76. Mean heterozygosity of the six microsatellites is 0.71 and mean PIC is 0.66.Thus it can be seen that the microsatellites are medial or high polymorphic loci.The correlation between the microsatellite markers and pork quality traits were analysed by SAS-GLM procedure. The results showed that there were significant correlation between microsatellite GAD (11323 ), 80149(10819) and pH value, bewteen S0088 and tenderness , WHC (extremely significant), extremely significant between S0284 and pH value, WHC. The analysis by LSD of one-way ANOVA in SPSS on those microsatellites significant correlative with pork quality traits shows that there is significant difference between the genotypes of 4 microsatellites. Thus these microsatellitesmay be referenced as the markers for further breeding of Sutai pig.