Polymorphism Of The Parental Linees In Hybrid Rice (Oryza.satival.) By RAPD Markers

Twelve rice male sterile lines, 9 maintainerblines and 15 restorer lines were analyzed by RAPD with 10primers.altogether,106 fragments were generated,of which 81 detected polymoprphism among rice lines tested.Cluster analysis showed that all lines could be uniqeuely distinguished by RAPD marker.In order to obtain a stable RAPD reaction system, we optimized the reaction conditions based on experimental results. The results showed that the reaction system , which included 10~60ng DNA, 2.0mmoI/L Mg2+, 1.5~2.0mmol/L dNTPs, 1.2~2.01 mol/L random primers and 0.8~1.2U Taq polymerase, was good for our experiments. Ten primers, which produced repeatable, clear and rich amplified polymorphism in the RAPD analysis, were obtained by screening 40commercial primers. They are S227,S228,S229,S290,S358,S400,S490,S1405,S1422and S1492.Based on the pair-wise comparsons of amplification products the genetic distance was calculated using Nei&Li's genetic similarity coefficient and a dendrogram was constructed using an un-weighted pair group method with arithmetical averages (UPGMA),the result that the genetic similarity coefficient was very identical and the genetic difference of 36 rice lines was very small, their genetic polymoprphism was distinguishable,even the genetic backdrop among the male sterile lines and maintaier lines was very similarity,their genetic polymoprphism was also distinguishable.