Effect Of Different Nitrogen Forms And Ratioes On Carbon-Nitrogen Metablism Of Flue-Cured Tobacco Leaves

field and solution culture experiments were conducted with K326 from2002 to 2003 to study the effect of the rotted sesame cake fertilizer amino acid and different nitrogen forms and ratios on carbon-nitrogen metablism of flue-cured tobacco leaves in Henan Agricultural University and Key Discipline and Open Laboratory of Agricultural Biological and Engineering Technique.The results were as follows:1.The results of field experiment in 2002 showed it is more suitable the decomposing of sucrose and the transform from nitrogen-metablism to carbon-metablism in the latter of growing period of the flue-cured tobacco about SS(sucrose synthesis enzyme);It showed that T2 had kept highest in the experiment and that is to say,T2 were on great photosynthesis and on the carriage of carbohydrate in the latter of growing period of the flue-cured tobacco about SPS(sucrose phosphorus acid synthesis enzyme).Inorganic nitrogen have been kept higher in the tobacco leaves,when applying fully inorganic nitrogen,so the decomposing metablism of sucrose is weaker;Applying the rotted sesame cake fertilizer can make the activity of starch enzyme keep the highest level;But applying the rotted cake fertilizer in the latter of growing period of the flue-cured tobacco the activity of amylase had the lowest level;The rotted sesame cake fertilizer had been kept highest level,so it was more suitable to the increasing of the activity of NR for T4.The content of T4 about amino acid is always higher than fully inorganic nitrogen since it was transplanted 30d.It showed it was better to the accumulating of sugar of T4 in the tobacco leaves;T4 was better to the raising the content of amino acid reduced carbohydrate potassium soluble protein and the decreasing of the content of nicotine. 2 The results of the solution culture experiment in 2002 showed it was not only applying fully inorganic nitrogen but also the ratioes the rotted sesame cake fertilizer and inorganic nitrogen,the cntent of NO3-N was increasing along with the increasing of the ratioes of the applying NO3-N and the content of the NO3-N of T4 is higher than fully inorganic nitrogen. It showed that the rotted sesame cake fertilizer was to the absorbing of the content of NO3-N and worse to the absorbing of NH4-N;The activity of GS was highest;It was showed about the activity of SS SPS the ratioes of the NH4-N was higher,it was better to the synthesis of the sucrose. 3 The results of the solution culture experiment in 2003 showed that the ratioes of the different amino acid and NO3-N NH4-N can increasing the activity of amylase and GS.The activity content of the chemistry composition had no more difference.It showed substititeing amino acid for a part of fertilizer had no more difference to thechemistry composition in tobacco leaves.