ISSR Analysis Of Populus SP Located In Northeast

Populus is important tree. In this paper, ISSR marker technique is used to identify 70 clones of Populus that are planted in northeast China. The main results are introduced as follow:1. The best reaction program and amplification system for ISSR-PCR have been established to these materials, that is template DNA 20ng primer as RM dNTP0. 20mmol/L TaqDNAl.5U, Mg2+1.5mM.2. In this study, 12 arbitrary primers of ISSR marker are used to analyze genetic variation among 70 clones. 132 loci are detected in total. The percentage of polymorphic loci varies from 37.12%to 59.85%, in which the lowest percentage belong to P. xiaohei X P. euphratina and the highest one is P. X euram. cv. serotina de poilan.3. The cluster of 70 clones in genetic relationship is constructed on the basics of genetic distance. The result demonstrates that 70 clones are clustered into two groups, respectively. The first group contains all varieties of P. alba or P. tomentosa, the second group is divided into two subgroups, in which one is mostly composed of the clones from hybrid of domestic varieties, and the other is gathered with foreign clones of P X euramericana.4. The results proved that ISSR marker technique could be used to identify the clones of Populus.