Studies On Polymorphisms Of H-FABP Gene And The Association With Intramuscular Fat Content In Pigs

The study mainly performed two Chinese native breeds: Dahe and Yanan, and two crossbred pigs: Dawu and DLY, which have relatively high IMF content. The genetic variation in 5'-upstream region and the second intron of H-FABP were detected by PCR-RFLPs, in addition, we also studied the effect of H-FABP genes on intramuscular fat (IMF) in these four breeds.We investigated the polymorphisms of H-FABP gene and the frequencies of gene and genotype. For Hinf I -RFLP, there were polymorphisma in these four breeds. The frequencies of H allele for Hinf I -RFLP in Dahe, Dawu, Yanan, and DLY, were 0.8000, 0.5000, 0.5000, and 0.9650, respectively. For Hinf* I -RFLP, there were no polymorphisms in the other three breeds except DLY. The frequencies of B allele in DLY were 0.1960. For Msp I -RFLP, there were polymorphisms in these four breeds. The frequencies of A allele in Dahe, Dawu, Yanan, and DLY, were 0.8000, 0.2500, 0.9430, and 0.7960. For Haelll-RFLP, there were polymorphisms in the other breeds except Yanan, which only had DD. The frequentcies of D allele in Dahe, Dawu, and DLY, were 0.9500, 0.5000, and 0.1830.For Hinf I -RFLP, DLY had low polymorphism (PIC < 0.25), the other breeds had intermediate polymorphism (0.25 < PIC < 0.5). For Hinf* I -RFLP, DLY had low polymorphism (PIC <0.25), and others' PIC were zero. For Msp I -RFLP, Dahe and Dawu had had intermediate polymorphism (0.25 < PIC < 0.5), Yanan and DLY had low polymorphism (PIC<0.25). For Haelll-RFLP, Dahe and DLY had low polymorphism (PIC<0.25), the other two breeds had intermediate polymorphism (0.250.05), while the other three breeds were no polymorphism. For Msp I -RFLP, the means of IMFwere no detectable effets between the genotypes in Dahe, Dawu, and Yanan (P>0.05). But it had high significant difference between AA (IMF content was 1.9639?.7317) and Aa (IMF content was 2.5510?.7793) genotype (P<0.01). For Hae III-RFLP, there were no polymorphism in Yanan and no significant difference between the genotypes in the other three breeds (P> 0.05).In the analysis of the correlation between H-FABP gene and the IMF content by using the data of these four breeds totally, for Hinf I -RFLP, the results showed that IMF content had the inclination of hh>Hh>HH. IMF content had high significant difference between hh (IMF content was 5.1134?.6847) and HH (IMF content was 2.5590?1.5459) genotype (P<0.01). For Hinf* I -RFLP, MFcontent had the inclination of bb>Bb>BB. There were no significant difference between the three different genotypes (P>0.05). For Msp I -RFLP, IMF content had the inclination of aa>Aa>AA. There were high significant difference between AA (IMF content was 3.0206?.2570) and aa (IMF content was 4.6710?.5365) genotype (P<0.01). For Hae III-RFLP, IMF content had the inclination of DD>Dd>dd. There were high significant difference between DD (IMF content was 4.9436?.4646), Dd (IMF content was 2.6007?.5084), and dd (IMF content was 2.1604?.9897) genotype (P<0.01).The effect of RFLPs for H-FABP gene on IMFcontent was analyzed by Least Square Means. IMF content of the monoploid genotypes hh/bb/AA/DD (Hinf I -hh, Hinf* I -bb, Msp I -AA, and Haelll-DD) is the highest (IMF content was 5.7157?.9225). The LSM valua of genotype hh/bb/AA/DD was the highest and it had the topmost effect. It's the best genotype.