Extraction,Isolation And Identification Of Flavones In Seabuckthorn (Hippophae Rhamnoides L.) Fruits

Extraction and isolation and identification of flavones in seabuckthorn (Hippophae rhamnoides L.) fruits were studied in this paper. Flavones of Hippophae rhamnoides have been at abroad , 32 flavones extracted and identified, but there were a few reports studies on Flavones of Hippophae rhamnoides at home, so it is necessary to extensive study about extraction, isolation and identification of flavones.Three compounds were isolated from the fruits of seabuckthorn with the extraction of organic (MeOH 5: 5: 3: 3: 1) solvent and with of isolation of polyamide chromatogramphic column (80-100) and high performance liquid chromatogramphy. On the basis of physical constants, chemical evidence and spectrum analysis[UV, MS, NMR (including 1H-NMR, 13C-NMR) ] , compound I basic structure is Quecertin, compounds II, III basic structure are Tamarixetin.three compounds (I, II, III) were identified. They are Quecertin 3-O-rutinose(I), Tamarixetin 3-O-glucoside(II)and Tamarixetin 3-0- P -D-glucoside 7-O- a -L-rhamnoside(III) Compounds II, III were identified from Hippophae .L for the first time and confirmed as new compounds by searching Chemistry Abstract (CA) .Three new flavones compounds were isolated from the fruits of(Hippophae rhamnoides L. subsp. Mongolia Rousi). This provides scientific basis for flavones compounds spectrum identification.Flavones compounds are extensive existence in the plants , Flavones of Hippophae rhamnoides have been regarded as the principal components responsible for the pharmacological activity of Hippophae rhamnoides fruits, Flavones of Hippophae rhamnoides have been studied in relation to a wide range of physiological functions such as inhibition of low density lipoprotein oxidation, hypercholesterolemia, antioxidant, antidecrepitude, antiulcer, antivirus , anticancer and so on by the many reports on the pharmacological, chemical, biochemical studies. This study provides a scientific basis for extracting, isolating, purificating, identifying on flavones of Hippophae rhamnoides h., in order to further to exploite and utilize seabuckthorn resource, especially provides new method for Flavones of Hippophae rhamnoides medicine industrialization production .