| For the purpose of Begonia elatior :micropropagation technology applying to the production, this paper studied on simplifying and optimizing the hormone combination of media and culture technique . The results are as follows:1. Establishment of aseptic explant culture system: About 1 cm long blade was cut; and the twice sterilization method was adopted to improve the sterilized efficiency and survival rate. Then the blade was inoculated on the improved culture medium. After 7 days'culture , it was found that the pollution rate of the material is less than 10%, but the survival rate is up to 100%.2.Callus and adventitious bud induction :The leave were cultured under the sunlight by using the improved liquid medium , MS+BA1+2, 4-D0.5. After two weeks, the bud differentiation rate was more than 1300%.3.Multiplying the intermediate propagator:The tin-bottles with flat bottom was filled with medium; sealed up with Kraft, which was covered with polyethylene membrane. Then divided the material.with tweezers in the bottle. The propagator was transferred into the improved medium(l/2MS+NAA0.1) to multiply. Through the high temperature treatment of summer , the shoots were disinfectedand the resistance character of flower seedlings were improved . After 4 weekss' culture , a explant was multiplied up to 10.times .4.The obtaining of strong shoots and their rooting: The shootl with bud was cut and inoculated on the culture medium (1/2MS+NAA0.1), then, sealed up with polyethylene membrane. It was laid in stronger natural light to grow. After two weeks, the rooting rate is up to 100%.5.Transplantation of plantlets: The plantlets were planted in humus after the roots were cleaned, then they were covered with plastic film. The relative humidity was kept over 80%. When laid in shade for a week, the plastic film was removed. Step by step, it was cultivated in the stronger light. After half of a month, the survival rate is up to 100%.
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