Studies On Technologies Of Tissue Culture Of Begonia Boliviensis And Juglans Mandshurica Var. Sachalinensis

Choosing two kinds of plants that were difficult proliferation including begonia’Crackling Fire(?)(Begonia boliviensis) and Japanese walnut (Juglans mandshurica var. sachalinensis). Considering the low production efficiency, diffficluty to maintain the stability of variety, contamination and browning in tissue culture, we did the research of begonia’Crackling Fire(?)’ and Japanese walnut by tissue culture. The results were as follows:1Technology development of begonia’Crackling Fire(?)’ tissue culture(1) For the4varieties of begonia’Crackling Fire(?)’, the optimal sterilization way is, after washing with neutral detergent and rinsing, the explants were surface sterilized with ethanol (70%,60sec) and then sterilized by10min immersion in1%sodium hypochlorite (NaClO) solution with Tween20solution. They were rinsed4times with sterile water then transferred to petri dish with the culture media.(2) For primary culture of begonia’Crackling Fire(?)’, the optimal medium was1/2N MS+TDZ10.0μM+NAA0.1μM+agar7g/L+sucrose30g/L.(3) In subculture of begonia’Crackling Fire(?)’, for B. boliviensis var. sunjirapi, B. boliviensis var. sunjiracrem and B. boliviensis var. sunjiraore, the optimal medium was1/2N MS+TDZ10.0μM+NAA0.1μM+agar7g/L+sucrose30g/L.(4) In rooting media, for B. boliviensis var. sunjirapi, B. boliviensis var. sunjiracrem and B. boliviensis var. sunjiraore, the optimal media was1/2N MS+CPPU0.1μM+NAA0.1μM+agar7g/L+sucrose30g/L. The rooting rate is100%.(5) For B. boliviensis var. sunjirapi, B. boliviensis var. sunjiracrem and B. boliviensis var. sunjiraore, the higheset way of propagation was to use one adventititous bud as subculture explant. The regeneration rate is10.5,10.1and14.8.2Technology development of Japanese walnut tissue culture(1) For Japanese walnut (Juglans mandshurica var. sachalinensis), the optimal sterilization way is, after washing with neutral detergent and rinsing, the explants were surface sterilized with ethanol (70%,60sec) and then sterilized by12min immersion in1%sodium hypochlorite (NaClO) solution with Tween20solution. They were rinsed4times with sterile water then transferred to petri dish with the culture media.(2) For primary culture, the optimal medium were WPM+TDZ1.0μM+Gelrite2g/L+Sucrose30g/L and WPM+Zeatin10.0μM+Gelrite2g/L+Sucrose30g/L. To add Vc5.0mg/L to decrease the browning of explants. The pH was adjusted to5.8.(3) For subculture, in media containing with TDZ, the adventitious buds differentiation were better and had complete leaves. In media containing with Zeatin, it inducted more callus.