Studies On Ultra-dry Storage And PEG Priming Methods Of Turfgrass Seeds

Eight Turfgrass Seeds were used as materials in the experiment, which were Festuca ovina (Jaguar 3 (1),Jaguar 3 (2),Total and Arid 3),Lolium perenne (Accent),Poa pratensis (Park)and Cynodon dactylon (Unhulled and Hulled).They were tested by ways of ultra-dry storage and seed priming. The changes of different vigor indexes of Turfgrass seeds were analyzed before and after storage, and Suitable ultra-low moisture value and low moisture value of different Turfgrass seeds were found. Through studying the effect of PEG priming on germination energy and vigor of Turfgrass seeds, suitable methods of PEG priming were found. 1.Turfgrass seeds were dried by discolored silica gel as drier, and different moisture grades of eight Turfgrass seeds were got. Two materials of each moisture grade were fetched, put, sealed and preserved for six months under normal temperature and low temperature (0~-5℃) separately. Turfgrass seeds before and after storage were tested by ways of seedling growth test, accelerated ageing test, cold test, electrical conductivity test, simulated field test, activation test of POD, CAT,SOD,TTC and APA and MDA content test. Compared the difference of different vigor indexes before and after storage, the results showed that suitable low moisture value or ultra-low moisture value of eight Turfgrass seeds were found in normal temperature storage and low temperature storage, and there were differences among the varieties. On the basis of the experiment,the preliminary results were as follows: Suitable low moisture and ultra-low moisture of Jaguar 3 (1) under normal temperature storage were separately 8.86% and 4.66%, and suitable low moisture and ultra-low moisture of low temperature storage were separately 8.86% and 3.56%; Suitable low moisture and ultra-low moisture of Jaguar 3 (2) under normal temperature storage and low temperature storage were the same, and they were 8.96% and 4.94% separately; Suitable ultra-low moisture of normal temperature storage of Total was 4.64%,and suitable low moisture of low temperature storage was 9.53%; Suitable low moisture of normal temperature storage of Arid 3 was 8.38%,and suitable ultra-low moisture of low temperature storage was 3.74%; Suitable low moisture and ultra-low moisture of Accent under normal temperature storage were separately 8.51% and 4.98%, and suitable ultra-low moisture of low temperature storage was 4.98%; Suitable ultra-low moisture of Park under normal temperature storage and low temperature storage was 3.38%; Suitable ultra-low moisture of normal temperature storage of Unhulled was 4.94%, and suitable low moisture and ultra-low moisture of low temperature storage were separately 7.87% and 3.52%; Suitable low moisture and ultra-low moisture of Hulled under normal temperature storage were separately 6.97% and 3.87%, and suitable ultra-low moisture of low temperature storage was 3.87%. 2.The result of regression analysis showed that through seedling growth test, accelerated ageing test and cold test, some of vigor indexes were not significantly correlated with seedling rate of simulated field condition, and other were highly significantly correlated positively with seedling rate of simulated field condition such as germination energy, sprout length, sprout fresh weight, sprout dry weight and simple vigor index. These showed that seedling growth test, accelerated ageing test and cold test were all better test methods for vigor test of Turfgrass seeds. Both electrical conductivity and absolute conductivity were highly significantly correlated positively with seedling rate of simulated field condition, and relative conductivity was significantly correlated positively with seedling rate of simulated field condition. The activations of POD, CAT, TTC and APA were highly significantly correlated positively with seedling rate of simulated field condition, while SOD activation was not significantly correlated with seedling rate of simulated field condition, and MDA content was significantly correlated positively with seedling rate of simulated field...