Cluster Analysis Of Morphologic Characters In Bowllotus Cultivars And Study On Them With Rapd Marker Technology

Lotus, an ancient plant, is one of the earlier plant species derived from angiosperms. It is a dicotyledon plant in spite of some monocotyledonous charaters. Studies based on embryo genesis show that, not only lotus plumule has special structure, but also the genesis of chloroplast develops a different pathway from others indicating that lotus might have an unique position in phylogeny of the angiosperm.As the development of the technology of the breeding and people's demand of viewing and admire are increasing constantly, more and more bowl lotus cultivars begin to appear , and have abundant hereditary characteristics. The study selected nine quantitative and eleven qualitative characters to cluster 23 bowl lotus cultivars. And carried on RAPD(Random Amplified Polymorphism DNA) analyse to some cultivars, found out one suitable amplification reation conditions for bowl lotus RAPD. RAPD marker technology had offered a new way for seed selection and qualification of further research for new bowl lotus cultivars in the molecular level. The concrete results are as follow.1. Cluster analysis of morphologic characters divided the 23 cultivars into three groups. Fifteen cultivars made up Group â… , and Group â…¡ and Group â…¢ comprised three and five cultivars respectively. Group I was divided three species by two lines : the red, the white and the yellow. Petals of the twenty-first cultivary reached 1000, with other hereditary distance of cultivars being relatively far. From the analysis result we could find out that the petal count, the color of petal, the size and the relation, etc. occupied the important status in the classification of small lotus. The result is almost same as it received with the traditional categorized method.2. Genomic DNA was successfully extracted from the leaves of bowl lotus with the modified CTAB method. The A₂₆₀/A₂₈₀ of genomic DNA extracted was 1.8-2.0 and the molecular weight was almost 23kb. It was fit for the further research and RAPD analysis.3. Suitable amplification reaction conditions for bowl lotus RAPD was established as total volum of 25μl containing 1.5 mM mg²⁺, 0.2mM dNTP Mixture, 0.15 U/μl Taq polymerase, 0.4nM primer and 40ng/μl DNA. The proper RAPD program was as follows:...