Study On The Culture Character And The Physiological Activesubstance Of Flammulina Sp.

100 thousand of fungi exist in china in which 300 kinds have medical value and only 30 kinds were studied, so the available potential of fungi is prodigious. The resource of fungi should be opened up to meet the medical and edible needs of people. We separated a kind of fungus in mulberry to carry through propagating and pertinent researches. The result are :A kind of Fungus named Flammulina sp has been gotten through tissue and single spore separation , which belongs to Basidiomycotina Hymenomycetes Agaricaels Tricholomaceae Flammulina. Meanwhile many characteristic was studied, such as: the culture character , biology characteristic; the extraction of amylose, and its biological function.Data showed that the fungus can grow a wide range of temperature from 6°C to 30℃, and the temperature from 22℃ to 26℃ is most suitable for growth. The hypha grow most rapidly in PDA culture medium at 26℃, but the aerial hypha is whiter and thicker at 22℃ while it show light yellow and shrink at higher temperature. So this kind of fungus is more suitable to grow at lower temperature. The fungus can grow in pH4-9 and fastest in pH 7 and grow in 10 kinds of culture mediums among which the mycelium grow more flourishing in soybean sprout culture medium and the same to mycelium in bran culture medium.A great deal mycelium was got through the vibrant liquid culture medium at 22℃, 130r/min. The mycelium was rubbed into powder after vacuum freeze-dry, then the powder was soaked in distilled water anddistilled in microwave oven, after ethanol deposition, removing fat and protein from deposition through the sevag's method, the coarse amylose was gotten by the vacuum desiccation. The amount of amylose through microwave method is 6%. The quality and quantity of the pure amylose was mensurated by the vitriol-phenol method and the content takes up 17% of coarse amylose, so the content of pure amylose in Flammulina sp is 1.02%. The main component of the amylose is glucose through paper chromatograph.In vitro culture cell, the different concentration amylose liquid of Flammulina sp impacted on Sf9 cell growth and inhibited the virus being observed. The result shows: the 0.25mg/ml concentration amylose liquid of the Flammulina sp is innocuity to the Sf9 cell and can inhibit the AcMNPV partially, while the 0.5mg/ml concentration amylose liquid of the fungus can restrain the growth of Sf9 cell.