Study On The Production, Absorption And Utilization Of Acetate And Butyrate In The Rumen Of Sheep And The Measurement Of Dietary Metabolizable Lipogenous Substances (MLS)

This paper consisted of two parts:Part1: Study on the production, absorption and utilization of acetate and butyrate in the rumen of sheep and measurement of dietary MLSExp1: The production and absorption of acetate and butyrate in the rumen of sheep and the measurement of MLSSix Inner Mongolian semifine-wool wethers (1.5 old years, 29-29.2 kg BW) fed a 1.0 M basal diet and fitted with a ruminal and a duodenum cannula were used to study the production, absorption, passage and utilization of acetate and butyrate in the rumen by simulating continuous feeding and pulse-continuous infusion technology. Rumen steady state was obtained through eight equal portions feeding daily, and dietary metabolizable lipogenous substances (MLS) was calculated subsequently. The results showed that: (1) Under the basal diet condition , the principal production rate of acetate and butyrate in the rumen of sheep were 61.66 mmol/h and 23.04 mmol/h respectively; the absorption rate of acetate and butyrate were 47.86 mmol/h and 17.86 mmol/h respectively; the passage rate of acetate and butyrate were 14.52 mmol/h and 5.19 mmol/h respectively; the fractional absorption rate of acetate and butyrate were 76.33% and 77.52% respectively. (2) the regression equation between the principal production rate (X, mmol/h)and the absorption rate of acetate and butyrate(Y, mmol/h) in the rumen of sheep were as follows: for acetate: Y=0.6905X+4.4554(R2=0.9973,n=5),for butyrate: Y=0.1514X+14.311(R2=0.8227,n=5);the regression equation between the production rate of acetate and butyrate(X, mmol/h) in the rumen of sheep and the rumen concentration of acetate and butyrate(Y, mmol/L) were as follows: for acetate: y = 0.3739x + 5.4456(R2 = 0.9899, n=5), for butyrate: y = 0.3617x + 1.8599(R2 = 0.9247,n=5.)(3)Dietary MLS and MG value were 135.69g and 43.83g respectively; the ratio of MLS/MG was 3.10; the ratio of C2+C4 energy and of C3+C6 energy in dietary ME were 89.24%和11.30% respectively.Exp2: The utilization of acetate and butyrate in the rumen in splanchnic tissues of the sheepThree inner Mongolian semifine-wool wethers (1.5 old years, 29-31kg BW) fed 1.0 M basal diet and fitted with chronic indwelling catheters in the mesenteric vein, hepatic...