Cloning And Expression Of BMP-2 Gene Fragments From Arbas Cashmere Goat And Mongolia Sheep

The cashmere comes from the second hair follicle of Cashmere goat. The quantity and quality of cashmere depend on the structures and characteristics of the hair follicle directly.Some signal molecule have relations to the development and circling the growth of hair follicle ,It's reported that BMP-2/4 controled by Noggin which is one of the signal molecule has relation to the second hair follicle of rat,but none of the reports about the relationship of BMP-2 with Cashmere goat and Mongolia sheep. The genome DNA were extracted from blood in Arbas cashmere goat and Mongolia sheep .The sequence were obtained by PCR and cloned into pMD18-T vector .The positive clones were sequenced.Finally we compared the sequences of BMP-2 gene fragments among goats,sheep and other animals that have been submitted to GeneBank. Aligment of the sequence of Arbas cashmere goat, Mongolia sheep with other animals showed that there was 99% nucleotide identity between Arbas cashmere and Mongolia sheep,87% identity between Cashmere and Human ,85% identity between Cashmere and Mouse, 99%identity between Cashmere and Cattle,86%identity between Cashmere and Dog;on the basis of comparing the sheep sequence to Human,Mouse,Cattle and Dog,the finding homology were 87%, 85%,99%, 85%.Then we made the probe with DIG using the cloning product of cashmere goat and Mongolia sheep,for studying the expression of BMP-2 in hair follicle of Arbas cashmere goat and Mongolia sheep by In situ hybrition. In cashmere goat ,we found the great expression in keratinization cell of the primary hair follicle in 75 days ,the great expression in the primary and the second hair follicle of 115 days; The expression in IRS at the primary hair follicle in March,the expression in the second hair follicle in May,the slight expression in the primary hair follicle in Oct; The expression in first hair follicle in Mongolia sheep in Nov.