Experimental Study On Chemical Constituents And Protective Effects Of Rhodiola

Rhodiola rosea L, a species belonging to genus in Crassulaceae of Rhodiola family, was firstly recorded as an herbal medicine in "The four medical Tantras" (Si Bu Yi Dian).Rhodiola rosea L grows in high latitudes of mountainous regio ns, or distributes in hills or waterside. Rhodiola rosea L is mild-nat ured, pucker, and tonifying kidney, which is usually used to treat the general fatigue, chest tightness, nausea, weakness and other sym ptoms.Rhodiola exhibited many biological activities, such as anti-agin g, anti-hypoxia, anti-antiviral, anti-tumor, anti-fatigue, what’s more,it provides protective effects on nervous system and regulates the bo dy to pharmacological actions bidirectionally.Various chemical constituents contained in Rhodiola were obtai ned, including coumarins, flavonoids, glycosides, alkaloids, amino ac ids, inorganic elements. Among them, salidroside and aglycones are the main effective components which are used as standard to eva luate the quality of Rhodiola. This study investigated the chemica 1 constituents of Rhodiola and the protective effect of salidroside to the damage of astrocytes induced by staurosporine, aiming to provi de experimental basis and research foundation for the in-depth study of the mechanism of neuroprotective effect of salidroside.The present study is focused on chemical constituents and anti oxidant activity of the ethyl acetate layer and n-butanol layer of ext racts of Rhodiola.on the system of the 70% ethanol.To obtain the p henylethanoid glycosides separated from Rhodiola, a variety of mod ern chromatographic techniques, such as silica gel column chromato graphy separation, were applied to separate and purificate Rhodiola according to the characteristics of the compound ethyl acetate extrac t parts. DPPH method was used to evaluate the antioxidant activity of monomeric compound. Eight compounds were systematically iso lated from the ethyl acetate extract of Rhodiola by silica gel, includ ing:eighteen acid, twenty-six acid, twenty-eight acid, β-Sitosterol, g allic acid, ethyl benzoate, salidroside, isoflavone.. DPPH assay result s showed that Rhodiola phenylethanoid glycosides existed in the ch emical composition of sitosterol, gallic acid, ethyl benzoate, salidros ide, isoflavones exhibited antioxidant activity.Our present study investigated whether salidroside has protectiv e and antioxidative effects on astrocytes. WST-1 assay was carried out to assess the cell viability of astrocytes and cortical neurons un der the treatment of the purified (>95%) salidroside. Moreover, WS T-1 assay was also used to evaluate the cytoprotective effects of sa lidroside in preventing astrocytes from staurosporine-induced cell de ath.Our results show that Salidroside can not only inhibit the releas e of reactive oxygen species(ROS) induced by agent STS, but also can effectively reduce the spontaneous release of ROS in cell, whic h indicates that the antioxidant effect of salidroside might be the m echanism of the protective effects to astrocytes.This research clarified the antioxidant activity of chemical cons tituents of Rhodiola and the protective effects of salidroside to astro cytes, then laid a solid experimental basis and theoretical basis for the the in-depth study of the mechanism of neuroprotective effects, clinical application, reasonable development and utilization of Rhodi ola resources.