Study On The Propagation System Of Virus-Free And Industry Development Of Potato

The major and efficient way to promote potato yield and control the degradation of potato seed tubers in the world is to use the virus-free seed tubers propagated via shoot meristem culture. Based on the virus-free seed tuber propagation system, this technology was spread in China in 1970's and gradually use to establish the propagation system that suited various montanic zones in China. However, these propagation systems conventionally exceeded five progenies in field and the quality of propagated tubers was not ensured due to incomplete virus detecting system in China. Along with the development of high-efficiency and low-cost propagation technologies, reducing the years of potato seed tuber propagation is the developmental tendency of seed tuber system. Two-year system plays important roles in reduction of seed tuber propagation period and lowering its production cost, facilitating application of new potato cultivars with market demands and enhancement of seed tuber quality.Based on the status of potato production and four-year potato tuber seed propagation system in Pan county, and stable and rapid propagation technology, the present research was carried out to study the high-efficiency and low-cost propagation technologies related to rapid propagation of potato plantlets, minituber propagation in web-house and production of minitubers in field with aims to reducing the production period of virus-free seed tuber. Also, combined with developmental status of potato industries in Pan county, the present study analyze the important roles of two-year seed tuber propagation system in potato industries of Pan county. The results were as followings:1. Rapid propagation of virus-free plant in vitro with high-efficiency and low-cost. The cost of tissue cultured plants were remarkably lowed through improvement of synthetical approaches: the distilled water used in tissue culture was replaced by common tap water, sucrose (analytic purity) by white sugar sell in market, artificial light by natural one and agar solidified medium by liquid superficial one. In this procedure, one nodule was retained at the base of mother potato plant in every propagation cycle.2. Low-cost in minituber production. In minituber propagation system, the density of truncated plantlets was controlled at 350/m~2 with broad row (10cm) and narrow row (4cm) and the distance between two plants 4cm in high humidity in Liu Pan Shui Gui Zhou provience. The density of shoot tips of plantlets was controlled at 350/m~2 with the same parameters mentioned above except for narrow row 4.8cm. Fertilization was carried out at grooves in broad rows to save the artificial cost of nutrient liquid irrigation. The cost of minituber production was reduced from 0.14 yuan to 0.06 yuan through deep plantation of plantlets, continuous yield of tubers and two harvests of minitubers from one time planted...