Study On Polymorphisms Of Myostatin Gene In Sheep

Myostatin is a screted protein that acts as a negative regulator in skeletal muscle mass .In this study, we analysis polymorphism of partial flanking sequence and coding region of MSTN gene in different sheep breeds, including German Merino, Texel, Doxset, BaShiBai, South Africa Merino and Duolang sheep.As a result, a G→A mutation was identified at +84(relative to translational initiation site) in German Merino. Two genotypes were found, and the genotype frequencies for GG and GA were 0.6944 and 0.3056 respectively. The mutation is a synonymous mutation and no polymorphism was detected within this region in other breeds.At the same time, four SNPs were found within exon3 of MSTN gene in xinjiang indigenous BaShiBai sheep.A T/C transition at nucleotide position+4977(relative to ATG) results in the substitution of a Lys by a Arg ; A T/C transition at nucleotide position+4892(relative to ATG)results in the substitution of a Thr by a Ala; A A/G transition at nucleotide position+5012(relative to ATG) results in the substitution of a Phe by a Leu; a A T/C transition at nucleotide position+4859(relative to ATG), results in the substitution of a Arg by a Gly. No polymorphism was detected within this region in other breeds.No polymorphism was detected within promoter, exon2 and 3'-UTR in all sheep breeds.