Tissue Culture And Rapid Propagation Of Acer Ginnala

The 10-year-old seedlings of Acer ginnala were used as the materials to study all kinds of factors that influenced the stem segment culture in vitro of Acer ginnala, such as explants disinfection, browning inhibition, screening of the media for primary culture and secondary culture, the optimal hormone concentration combination for secondary culture , the optimum rooting medium and phytohormone concentration, proportioning and selection of rarity etc. The results were as follows:1. April was the best period of picking the explants. The disinfection efficiency of top bud was better than the others. The most optimal method of explants disinfection was disinfecting their surface by 70% alcohol about 30 s , thereafter washing 3～5 times in aseptic water, then dipping them in 0.1% HgCl 2 about 7 min, finally washing 3～5 times in aseptic water again.2. The tender stem segments in April as explants were relatively low in browning and mortality rate about 35.8% and 37.5%. Glucose was more suitable than saccharose as the carbon source of anti-browning culture medium. 1/2 MS medium could alleviate explants browning. Adding 1.0mg/L PVP and 2.0g/L AC in the medium could prevent explants browning effectively. But when 2.0g/L AC was the antioxidant, explants callus generated lower.3. The optimal medium for germination culture was MS+0.1mg/L NAA+7.0g/L Agar+30g/L saccharose. The germination rate of bud induction was up to 77.0%. The germination induction rate of stem with buds as explants was higher than the rate of top and lateral bud.4. The medium MS was more suitable than 1/2MS, WPM and B5 for callus induction from stem segments. The induction rate reached 79.0%. The optimal medium for callus proliferation was MS+1.0mg/L NAA+0.1mg/L BA +0.5mg/L IBA. The proliferation rate got to 99.0%. But the callus hardly had differentiation. Further research of reasons were expected.5. The optimal proliferation medium for stem segments was MS+0.5mg/L NAA+2.0mg/L BA. The proliferation rate was 93.0%. The proliferation rate of MS+0.2mg/L NAA+2.0mg/L 6-BA+1.0mg/L IBA next to forward was 90%.6. The macroelements had obvious effect on rooting rate. The medium of 1/2MS+1.0mg/L IBA+30g/L and WPM+1.0mg/L IBA +20g/L saccharose were suitable for rooting and the rate was up to 90%.7. Using the peat soil to be the matrix material, after 30d transplantation, the survival rate reached 87.5%.