Study Of The Toxicity To Pests By The Compound Of Root Of Stellera Chamaejasme L.

There are many components in the root of Stellera chamaejasme L., however, the poison of the pure Stellera chamaejasme L. is not good as the compounds. There are some active components in the root of Stellera chamaejasme L., therefore, in this study, the root was extracted and combined with other botanic pesticides as a new effective botanic pesticide. After that, we isolated and checked the high active material element as the standard of quality control.It is the first time to combine the different botanic pesticide with the extracts of Stellera chamaejasme L., and it is found that there is high toxicity of combination to Petrbia harti Ewing, Oleanders aphids, Panonychus citri, Tetranychus urticae Koch and housefly. Comparing with the single Stellera chamaejasme L.'s extract from Soxhlet Method, the toxicity of the combination active fraction from the roots of S. chamaejasme's extracts (LD), with nicotine oil emulsion (YJR) or/and CT-901A (Bz), which is a kind of synergist in pesticide, to Petrbia harti Ewing are about 1.130,1.126,1.128 times respectively, and, to housefly are about 1.219,1.108,1.256 times separately.Refer to the past research and experiments, the active poisonous materials in Stellera chamaejasme L. are mostly in the extract from Sohxlet method with petroleum ether, therefore, we dipped the root in ethanol in heat and stirring, at the meanwhile, the components which extracted by ethanol were extracted by Sohxlet extraction with petroleum ether(60～90℃). Then LD was separated and purified by silicagel column chromatography, after that the active material in Stellera charnaejasme L. was tested with toxicity mensuration. Through isolation in column, I got three fractions with highly poison, which are in the nonpolarity fractions of extract, tested by 3% FeCl₃ ethanol solution—specific visualization reagent of flavonoids—as visualization reagent in LD's thinlayer chromatography. It is deduced that the active material in S. chamaejasme's extract is flavonoids, according to the departed researches.Basing on the high poison in LD, in my research, I compounded the LD with other pharmacopoeia, which is relative pesticide. Therefore, by toxicity mensuration interior, the results showed that synergistic effects were observed in all combination of LD+Bz, LD+YJR and LD+Bz+YJR. The toxicity of the combination active fraction from the roots of S. chamaejasme's extracts (LD) with nicotine oil emulsion (YJR) or/and CT-901A(Bz) different mixture was studied on housefly and Petrbia harti Ewing. The toxicity regression analysis, LC₅₀ and regression equation were obtained by using EXCEL. The results showed that synergistic effects were observed in all combination of LD+Bz, LD+YJR and LD+Bz+YJR. Both the LC₅₀ of 20:1:1(LD:YJR:Bz), 30:1:1(LD:YJR:Bz) on housefly, and the LC₅₀ of 20:1(LD:Bz), 30:1(LD:Bz), 30:1:1(LD:YJR:Bz) on Petrbia harti Ewing were 0.005 mg L^-1. Moreover, the active fraction of S. chamaejasme's roots extracts was isolated initially by column chromatography, and the active compounds were flavonoids. It advanced the poison effects of StelIera chamaejasme, and improved the application of botanical pesticide from root of Stellera chamaejasme.