Tissue Culture Of Pueraria Thomsonii Benth. And Study On The Effect Of Irradiation On Tube Seedling

Radix Puerariae, the dry root of Pueraria thomsonii Benth., is one of traditional Chinese medicines. In recent years, Radix Puerariae powder, whose comprehensive value in use has been improved gradually became significant officinal and raw material for industry production. In this study, we focused our attention on the establishment of rapid propagation system and the induction condition of the tube earthnut, and also the effect of 60Coγray irradiation on the growth of cultured seedlings of Radix Puerariae. The results are listed as follow:1. Study on the establishment of rapid propagation system of Radix Puerariae.The dormancy of Radix Puerariae.seeds is caused by the imperviousness of seed coat, which can be smashed by machinery break and reached 80% of the germination rate . Sterilized by 75% ethanol and 0.1% HgCl2 for 2 times can reduce the pollution rate to 15%. Propagation culture medium of tube seedling is MS+6-BA2.0mg/L+NAA0.1mg/L, by which the grpgation coefficient was 18%; sound seedling culture medium is MS+6-BA0.1mg/L+NAA0.1mg/L; root inducing culture medium is 1/2MS+IBA1.0mg/L, and the root gemination rate was 90%.2. Study on induction condition of the tube earthnut of Radix Puerariae.The optimum tube earthnut induction culture medium is MS+6-BA1.5mg/L+NAA0.1mg/L; high sucrose content and higher temperature were both beneficial for the increase of the tube earthnut diameter., and the most suitable sucrose content and the optimum temperature for the tube earthnut induction were 5% and 25℃respectively; exogenous GA3 went against the formation of tube earthnut.3. The impact of 60Coγray irradiation on the growth of Radix Puerariae tube seedlings.The rooting ability was inhibited as the dose ofγray increased, when the dose was up to 40Gy and with the treatment of 1Gy/min the rooting ability of tube seedlings lose; and the dose lower than 10Gyγray can increase the height and propagation of tube seedling;when the irradiation dose surpass 10Gy the growth can be inhibited, and the dose upper to 60Gy can absolutely stop the growth of tube seedling; the optimum dose for the Radix Puerariae tube seedlings is 20Gy with the treatment of 1Gy/min, the fatal dose was 100Gy with the treatment of 1Gy/min.