| Pesticides plays an irreplaceable role in improving agricultural production and in integrated pest control. It is widely applied in the field of agriculture. However, the irrational use of pesticides leads to seriously excessive residues in the environment and food and that is adverse to human health and environment. Therefore, our country is gradually establishing and perfecting a reliable, sensitive and practical pesticide residue analysis technology, to achieve a reasonable standard of the purpose of the reasonable use of pesticides.Gibberellin (GA3) is a kind of plant growth regulator pesticides. When GA3 is used excessively, it would produce a certain toxicity. In this paper, we established an analysis method detecting the GA3 residue in fruit and vegetables with high-performance liquid chromatography (HPLC) . The HPLC testing conditions has been optimized: Stationary phase is C18; UV detector and the detection wavelength is 254.00 nm; The mobile phase is 40% methanol-water solution which pH is 4.0; The method of Sample extraction, separation and purification has also been optimized: GA3 is extracted with 80% methanol-water solution by ultrasonic shock from samples; The extract is decoloried with petroleum ether and GA3 extracted with ethyl acetate, and then, RP-extracted with PBS and extracted with ethyl acetate again. Volatile the ethyl acetate in the extract by rotary evaporation and then dissolve the residue with methanol followed by being filtered with 0.45 um membrane, and this solution is test sample for HPLC.The method established in this paper has high sensitivity, precision and accuracy. The results is satisfying when it is used in detecting the GA3 of various fruits and vegetables. This method meets the pesticide residue testing standards that must be sensitive, accurate and easy to promote. So, this method has the most valuable promotion for detecting the GA3 Residues of various fruits and vegetables.
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