Study On The Yield Of Polysaccharide Of Pleurotus Eryngii Cultured In Liquid Condition

Pleurotus eryngii is a dual-use of Food and Drug fungal and rich in polysaccharide which has the effect of anti-cancer. But the artificial cultivation of its fruitbody meets many difficulty, such as the long production cycle, the limit of the season and weather, taking up a large amount of land , manpower, low output,high costs and many other adverse factors. Using liquid cultivation method to cultivate mycelia of Pleurotus eryngii, can shorten the production cycle, simplify production process and reduce costs. Therefore, the method for determining polysaccharide, liquid fermentation conditions in shake flask culture, the extraction techniques of polysaccharide in mycelium and extracelluar polysaccharide of x7, a strain of Pleurotus eryngii, were studied.The results were as follows: By comparison potatoes,corn powder, wheat bran three kinds of culture medias and Culture volume, inoculum quantity, the initial pH value,seed culture age to the production of mycelium, the mycelium and extracellular polysaccharide production, and the optimal medium culture was determined: wheat bran 5%, peptone 0.1%, sucrose 2.0%, MgSO4?7H2O 0.05%, KH2PO4 0.1%.To the mycelial growth as indicators, the fermentation conditions are as follws: culture volume was 100ml, inoculum quantity was 6%, initial pH value was 5.0, seed culture age was 4 days. To the mycelium polysaccharide as indicators, the fermentation conditions are as follws: culture volume was 100ml, inoculum quantity was 6%, initial pH value was 5.0, seed culture age was 4 days. To the extracellular polysaccharide as indicators, the fermentation conditions are as follws: culture volume was 100ml, inoculum quantity was 2%, initial pH value was 6.0, seed culture age was 4 days. Choice ultrasonic as a method of mycelium cell disruption. the optimum extraction temperature was 97℃, the optimum extraction time was 2.0h, the liquid-solid ratio was 8:1(ml/g). Isolated conditions of polysaccharide: three times the volume of 95% ethanol precipitation over 8 hours. Using Phenol-sulfuric acid method for determining polysaccharide, the detective wavelength was 485nm.