Hybridization Breeding Of Pleurotus Eryngii With Ultraviolet Irradiation On Mononuclear Mycelia And Selecting Of Filial Generations

Pleurotus eryngii is one of the important edible fungis with high value and medicinal properties. The industrialization and scale of Pleurotus eryngii is developed with the generalize of liquid spawn planting method. In this experiment, as the parent strain of Pleurotus eryngii, the application of submerged culture spawn for Xing-T was studied. with ultraviolet radiation mutagenesis and monospore cross breeding, the good quality progeny strains would be obtained by a series of selecting experiments.1. An optimum medium was determined, dand compositions of fermentation were as follows: Soybean powder 2%, wheat bran 2%, glucose 1%, yeast extract 0.5%, KH2PO4 0.1%, MgSO4·7H2O 0.1%, VB1 0.002%. The activity of pellets reached largest, and the fermentative period was 7 d. The optimal storage time of liquid spawn in 4℃was 6 d, and The time of the liquid spawn from germination to harvest was 51 d.2. In this experiment, with ultraviolet irradiation on mononuclear mycelia of Pleurotus eryngii, five different time stages were set up, and monospore cross breed with the mutagenize of mycelia.3. 76 new crossing progeniy strains were obtained by microscope checking and antagonistic experiment. And 16 new strains were obtained by means of vertical slab polyacrylamid gel electrophoresis and analysis of esterase isozyme of parent and new cross combinations, which had mutually complementary enzyme bands and new enzyme bands.4. Comparing the growth of mycelium, the resistance to Trichoderma Viride and characteristics of fruiting bodies, Z26 growed fast in the mother culture with 4.82 mm/d. Z56 was the best strain in the liquid medium with the highest weight, the smallest diameter and the largest density. The average biological efficiency of Z6 and Z25 was higher than parent strain.5. Four strains were obtained. The mycelium of Z26 growed well and faster, and had stronger resistance to Trichoderma Viride. Z42 and Z59 showed bigger fruiting body with well characteristics; Z56 was in order to liquid spawn.