Research On Relevance Of Serving Time Of Qinggong Drug And Its Clinical Efficacy

The Kunming mouse and Holstein cows were been taken as laboratory animals for this experiment,which study by randomized experimental design to study vitro uterine contractions of the mice during pregnancy,simulation estrus period,and1 to 9 h after childbirth;the changes of the level of E₂ and P₄ from 1 to 9 h within mice'delivery and1 to 6 days within cows' childbirth;to study taking Qinggong drug after childbirth in different level of E₂ and P₄ to increase uterine tension,reduce the number of bacteria in the uterus, promote uterine purification,and speed up recovery of related reproductive function.And discuss the best time of taking Qinggong medicine after cows' childbirth.1.The infuhence of Qinggong drugs on mice's vitro uterine contraction during pregnancy and analog estrus.We divide 32 baby mouse into A,B,C,D 4 groups of each have eight mouse.A is taken drug in 14-days' pregnancy.B is not taken drug in 14-days' pregnancy.C is taken drugs after estrus and D is not taken drugs after estrus.We determine vitro uterine contractions in one hour after the 32 mouse using the medicine,and the result showes that,uterine contraction in group A,B respectively significantly highter than in C, D(P＜0.05).2.The infuluence of taken Qinggong drugs after mice's childbirth on uterine contraction.80 female rats after childbirth are divided into A(use medicine after childbirth in 1h),B(use medicine after childbirth in 3h),C(use medicine after childbirth in 5h),D(use medicine after childbirth in 7h),E(use medicine after childbirth in 9h),F(not use medicine after childbirth in 1h),G(not use medicine after childbirth in 3h),H(not use medicine after childbirth 5h),I(not use medicine after childbirth in 7h),J(not use medicine after childbirth in 9h) 10 groups,each with 8 mouse.We determine vitro uterine contractions after take them medicine in one hour.Result shows that,dosing groups' uterine contractility are significantly highter than the otherwise(P＜0.05),and group d is obviously higher than group I(P＜0.01).3.The change of P₄ level in the mice,s blood after childbirth.40 female rats after childbirth are divided into A(not use medicine after childbirth in 1h),B(not use medicine after childbirth in 3h),C(not use medicine after childbirth in 5h),D(not use medicine after childbirth in 7h),E(not use medicine after childbirth in 9h) 5 groups,each with eight mouse.We determine each group' E₂ and P₄ levels in blood by RIA,and the result shows that,E₂ levels in the mice,s blood of group D is significantly highter than in group A,B,C and E(P＜0.01).4.The changes of E₂,P₄ level of the cow after childbirth.We select six cows randomly which are normal deliveries,and determine E₂,P₄ level in the 1,2,3,4,5,6 days after delivery.The results show that,E₂ has a upward trend in the first 1～3 days,and begins declining from the 4th day.P₄ in the first one to four days has a upward trend,and begins to decline from fifth days.5.The correlation among taking Qinggong drug after childbirth in different level of E₂ and P₄ in cows' blood,the number of bacteria in the uterus,and recovery of related reproductive function.We divide 48 cows which have normal delivery in the first 2 to 3 childbirths into six groups randomly,group A(take Oinggong drug after delivery in 1d), group B(take Qinggong drug after delivery in 2d),group C(take Oinggong drug after delivery in 3d),group D(take Qinggong drug after delivery in 4d),group E(take Qinggong drug after delivery in 5d),group F(not take Qinggong drug after delivery),and each group has 8 mouse.We respectively determine each group' total number of bacteria, number of lyma exhaustion's days,the rate of first breeding conception,the rate of endometritis,and estrus cycle of the 1～7 days after delivery.The result shows that:Delivery after the 7th day,the total number of bacteria in the cows uterus of group 6(5.45±0.42×10⁶ Cfu/ml) and group 1(5.59±0.23×10⁶ cfu/ml) significantly higher than the other groups(P＜0.01).The average days of lyma exhaustion(15.6 d) and the first estrus on group D is shortest in all groupes,but the difference is not significant.The first breeding conception rate on group D(80.00%) is significantly higher than that of a group A(40.00%) and group F(40.00%)(P＜0.01).The rate of occur endometritis on group D(0.00%) is significantly lower than group A (12.00%) and group F(37.50%)(P＜0.01). Normal estrous cycle rate on group D(100.00%)is higher than that of group A(80.00 percent) and group F(62.50 percent),but the difference is not significant(P＞0.05).This study shows that:the effect of takeing Qinggong drug is closely correlated with the level of E₂,P₄ in blood.We take cows Qinggong medincine on the fourth day after cow's delivery when E₂ starts to decline,P₄ begins to increase,and have good effect on obvious reproductive function for postpartum recovery,decreasing rate of endometritis and improving the first breeding pregnancy rate significantly.