The Huainan Chicken is high-quality varieties in Anhui,It is succulent flavor but slower growth.People were used to add the antibiotics to improve its growth rate,so a large number of antibiotics were residended in chicken body,that is endangering the health of people,but also different levels of environmental pollution.It has a serious impact on economic and social benefits of Huainan Chicken. To improve the immunity of Huainan Chicken growth performance and ensure the safety of their products.The experiment adopted the one factorial experiment design. In the experiment,280 huainan chicken were randomly devided into 4 groups of 70 chicken.The chicken in group one were fed a basal diet,the chicken in groupâ… were fed the basal diets plus 0.5%Chinese Herbal,the chicken in groupâ…¡were fed the basal diets plus 1%Chinese Herbal,the chicken in groupâ…¢were fed the basal diets plus 2%Chinese Herbal。The total growth time of is 42 days,and the Chinese Herbal was added to the feed for 35 consecutive days from days 7.At the 14th,24th,28th,35th and 45th day,we weighted the remaining feed,recorded the weight of consumption feed of every stage,the mining food for the calculation,the average daily increase of the Huainan Chicken avoirdupo is and feed gain ratio from 7:00 to 9:00 for each group.We collected 5 Huainan Chicken cardiac blood serum from each group for mensurate the anticoagulant of PHA-T lymphocyte and interleukin and the serum immunoglobulin levels,then we collected their spleen,thymus bursal and both sides of the thymus to weight.At the end of the trial,20 chicken(5 per group) were bled via the anterior vena cava.The proximal duodenum,jejunum,middle ileum were recollected and stored in 10%formalin solution immediately for preparing tissues section and measuring the number of intraepithelial lymphocyte. The results were as follows:1 The Compound Chinese Herbal can improve the growth performance of Huainan chicken significantly.Compared with the control group,AW of trial groups chicken in Chinese was higher than that of control significantly between d 21 and 28(p<0.05).AW in Chinese Herbal groupâ…¡andâ…¢were higher than that of the control group on d 35.(p<0.05). AW in Chinese native medicine groupâ…¡andâ…¢decreased significantly on d 45 (p<0.01)2 The compound Chinese medicine preparations can enhance the immune organ index of Huainan Chicken significantly.Compared with the control group,the difference of the spleen index of Groupâ…¡is significant on d28,35 and 42(p<0.05),the difference of the bursa index is significant on 21,28,35 and 42(p<0.05),the difference of the thymus index of each experiment Group is significant on d 35(p<0.05).3 The compound Chinese medicine preparations can enhance the immunoglobulin levels of Huainan Chicken significantly.Compared with the control group,the difference of the immunoglobulin level of Groupâ…¡is significant on d 21,28,35and42(p<0.05).4 The compound Chinese medicine preparations can enhance the serum protein level of Huainan Chicken significantly.The difference of the albumin level of each Group is not significant on d 14,21 and 35(p>0.05).Compared withthe control group,the difference of the Groupâ…¡is significant on d 28 and 42(p<0.05).5 The compound Chinese medicine preparations can enhance the NU-HI of Huainan Chicken significantly.Compared with the control group,the difference of the Groupâ…¡and Groupâ…¢is significant on d 21(p<0.05),the difference of the GroupI is not significant (1>0.05).Compared with the control group,the difference of the Groupâ…¡and Groupâ…¢is extremely significant on d 28 and 35(p<0.01),the difference of the Groupâ… ,Groupâ…¡and Groupâ…¢is significant high then the control group on d 42 (p<0.05).6 The compound Chinese medicine preparations can significantly enhance the T LCR of Huainan Chicken.The compound Chinese medicine preparations can enhance the T LCR of Huainan Chicken significantly at metaphase and anaphase of the experiment.Compared with the control group,the TLCR of the Groupâ… ,Groupâ…¡and Groupâ…¢is not significant (p>0.05) on d 14 and 21,the Groupâ… ,Groupâ…¡and Groupâ…¢is significant (p<0.05) on d 28,35 and 49.(p<0.05).7 The compound Chinese medicine preparations can enhance the WBC phagocytosis rate of Huainan Chicren significantly.The compound Chinese medicine preparations can enhance the WBC phagocytosis rate of Huainan Chicken significantly in the whole experiment period (p<0.05).Compared with the control group,the difference of the Groupâ… , Groupâ…¡and Groupâ…¢is significant(p<0.05) on d 21,28,35,42,the WBC phagocytosis rate of the Groupâ…¡is higher than Groupâ…¢and Groupâ… on d 21,28, 35 and 42.8 The compound Chinese medicine preparations can enhance the chicken blood antioxidase enzymatic active of Huainan Chicken significantly.Compared with the control group,the difference of Groupâ…¡is significant (p<0.05) on d 28,the difference of Groupâ…¡is extremely significant(p<0.01) on d 35.Compared with the groupâ… ,the difference of Groupâ…¡is significant (p<0.05) on d 35 and 42.9 The compound Chinese medicine preparations have a big influence on some hormone of Huainan Chicken.The GH of the Groupâ… ,Groupâ…¡and Groupâ…¢is lower than the control group.Compared with the control group,the difference of the Groupâ… ,Groupâ…¡and Groupâ…¢is significant(p<0.05) on d 21,the Groupâ…¡and Groupâ…¢is significant(p<0.05) on d 42.The T3 level of the Groupâ… ,Groupâ…¡and Groupâ…¢is higher than the control group.Compared with the control group,the difference of the Groupâ…¡is extremely significant(p<0.01),the difference of the Groupâ… and Groupâ…¢is significant(p<0.05),the difference of the Groupâ… ,Groupâ…¡and Groupâ…¢is significant(p<0.05) on d 42.10 The compound Chinese medicine preparations have a big influence on intestine mucosal morphology of Huainan Chicken.Duodenum:Compared with the control group,the difference of the villus length of each Group is significant(p<0.05),the difference of the crypt depth of Groupâ…¡is significant(p<0.05),the difference of the intestinal thickness of each Group is significant(p<0.05).Jejunum:Compared with the control group,the difference of the crypt depth of each Group is significant(p<0.05),the difference of the intestinal thickness of Groupâ…¡and Groupâ…¢is extremely significant(p<0.01),the difference of the Groupâ… is significant(p<0.05).Ileum:Compared with the control group,the difference of the villus length of Groupâ…¡and Groupâ…¢is significant(p<0.05),the difference of the intestinal thickness of Groupâ…¡and Groupâ…¢is significant(p<0.05),the difference of the Groupâ… is not significant(p>0.05). The test results showed that the compound of Traditional Chinese Medicine is significant on growth performance and immune properties of Huainan Chicken.It is not only for the healthy breeding and product safety provide valuable reference of Huainan Chicken but also for the further study of Chinese medicine formulations in the production of animal and provide a theoretical basis.
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