Isolation And Identification Of Chicken Coccidian And Study Of Chinese Medical Herb And Western Medicine Compounds For Preventing And Treating Chicken Coccidiosis

Chicken coccidiosis is caused by one kind or more kinds of Eimeria and happens worldwidely.The sickness causes harm to chicken seriously,which causes the huge economic loss in chickens industry.Chicken coccidiosis consists of a mixed infections caused by various coccidiosis,in which Eimeria tenella and E.necatrix's are stronger than others.This research separated Chicken coccidiosis from chicken's excrement in some areas of Anhui province.The identification of coccidiosis was carried by morphological method.Antibiotic experiment was carried on to find anti-coccidiosis medicine.According to the antibiotic nature test results,the Chicken coccidiosis biological characteristics and chicken's physiological characteristics,Chinese herb and antibiotic therapy method were develped on the basis of previous research of Chinese herb.The prevention effect on coccidiosis,and its effect on the disease were also studied.Firstly,chicken's fresh excrement was gathered from folowing counties in Anhui province,namely,Feidong,Feixi,Changfeng,Wuhe and Xuancheng.Oogonium were collected by the up-and-down methed,suspended with 2.5%potassium solution and incubated at 29℃.And then mutipled in Chicken.Gathered oogonium were observed using light microsope,The results showed that the seprated ooganium consisted of Eimeria necatrix,E.acervulina,E.maxima,E.tenella,E.mitis,E.hagani and E.praecox.All of them were included in Eimeria.Secondly,Sulfachloropyridazine pyrazine sodium,salinomycin,diclazuril, sulfaquinoxaline and Toltrazurila were tested for anti-coccidiosis effects.The results showed that the effect of Nicarbazin and sulfaquinoxaline were best.Thirdly,after combining sullfaquinoxaline,Nicarbazin,Madubamycin and amprolium respectively,anti-coccidiosis prescription was selected in chicken.The research was to choose choice proper time,dose and prescription of chinese herb and anti-biotic medicine for preventing and treating coccidiosis.The results showed that anti-coccidiosis index were up to 189 and 187 in preventing group and curing group respectively.Its quality met the pharmacopoeia standard of People's Republic of China in 2000.Fourthly,the experiment was carried out to prevent chiken coccidiosis using Chinese herb and western medicine compound prescription.We studied the change pattern of physiological biochemistry target in young chicken that was vaccinated after using Chinese herb and western medicine compound prescription.The results indicated that the content of GLU,Mg,Na,TP,ALB,SDH,AMS and ALP reduced significantly;and the content of Ca,NO,LZM,GOT and GPT increased,as well as IgG,IgM and IgA.But SOD,GU and K didn't change obviously.The level of GLU was maintained normally while LZM increased significantly.The Chinese and western medicine compound prescription increased obviously the chickling to grow and improved chickens industry and economic efficiency.In summary,this study carried on the epidemiology investigation successfully in some areas of Anhui province.We develop a compound prescription of chicken coccidiosis's Chinese herb and western medicine,which not only has the good anti-coccidiosis effect,but also can promote the chickling to grow,improve the feed conversion rate.Therefore,this research has explored a new choice for the chicken coccidiosis's prevention.It can enhance economic effciency in chiken industy.