| 87 tobacco samples from 5 typical ecological locations, 13 tobacco samples of Brazil, and 13 tobacco samples of Zimbabwe were studied. Routine chemical compositions, polyphenol, organic acid and volatile aroma components of 113 samples were determined. The distribution characters of the polyphenol in different latitudes were discussed. The difference of the phenolic compounds was analyzed among different stack positions, different latitudes and different altitudes by methods of analysis of variance. And the relationship between phenolic compounds and routine cheroical compositions, organic acid and volatile aroma components were analyzed by methods of analysis of correlation, cluster analysis and variance analysis. The results showed that:1. There existed large discreteness for the phenolic compounds in different ecological locations. The mean of phenolic compounds was 34.15mg/g, with the range of 0.06~0.66mg/g and the coefficient of variation 47.49%. The average of neochlorogenic, chlorogenic acids, quinic acid, scopoline, scopoletin, rutin and kaempferol-3-rutinoside was 2.49 mg/g, 15.92 mg/g, 3.39 mg/g, 0.21 mg/g, 0.22 mg/g, 10.93 mg/g, 0.99 mg/g, respectively. 7 phenolic compounds of green tea were tested. Chlorogenic acids had the highest content among them, reaching 46.63% of the total. The next was rutin, reaching 32.00% of the total. Aspartic acid, Serine, Methionine ect. Scopoline had the lowest content among them, only at present 0.60% of the total. The scopoletin was second lowest. The variation coefficient of chlorogenic acids was biggest (27.70%), conversely, The variation confficient of scopoletin was somallest (0.48%).2. There were significant differences of polyphenolic substances in different parts, different areas and different latitude, but no significant differences in different altitude. From the point of variability of different areas, the amount of polyphenolic substances had a low variation coefficients and higher stability. The tobacco in Yunnan and Dongbei had higher polyphenolic substances and lower in Fujian and Zimbabwe, the difference of this two groups reached a significance level of 0.05. The amount of polyphenolic substances in Guizhou, Shandong and Henan were at the upper level, not significant lower than Yunnan and Dongbei, significant higher than Fujian and Zimbabwe. The amount of polyphenolic substances in Brazil had a lower level, significant lower than Yunnan and Dongbei, not significant higher than Fujian and Zimbabwe. The polyphenolic substance amout of these 7 areas were: Yunnan > Dongbei > Guizhong > Shandong, Henan > Brazil > Zimbabwe.The polyphenolic substance amount in different parts were: upper-leaves (36.89mg/g) > lower-leaves (34.60mg/g) > middle-leaves (33.69mg/g); The amount of polyphenolic substances in middle-leaves were significant lower than upper-leaves, not significant difference with lower-leaves. The amount of polyphenolic substances in different altitude tobacco were: higher-altitude (37.70mg/g) > middle-altitude (35.60 mg/g) > lower-altitude (33.72mg/g), the coefficient of variation were: lower-altitude (17.07%) > middle-altitude (12.28%) > lower-altitude (7.94%); the polyphnolic substances of higher-altitude tobacco were significant higher than lower-altitude tobacco, not significant difference with middle-altitude tobacco.The amount of polyphenolic substances in different latitude were: middle-latitude (36.11mg/g) > higher-latitude (35.92mg/g) > lower-latitude (34.36mg/g), the vtariation coefficient were:lower-latitude(15.54%) > middle-latitude (14.68%) > higher-latitude (10.78%), the difference of polyphenolic substances in different latitude tobacco didn`t reached the level of significant.3. The studied between polyphenolic substances and main chemical composition indicates that neochlorogenic acid was significantly correlated with K/Cl and chlorine. Chlorogenic acid was significantly correlated with sugar-nicotine ratio, nitrogen-nicotine ratio and potassium; Quininic acid was significantly correlated with sugar-nicotine ratio, nitrogen-nicotine ratio and nicotine. Scopoline was significantly correlated with potassium and potassium-chlorine ratio. Scopoletin was significantly correlated with total nitrogen, potassium, total sugar and reducing sugar. Rutin was significantly correlated with nicotine, potassium, potassium-chlorine ratio; Kaempferol-3-rutinoside was significantly correlated with total sugar, reducing sugar, sugar-nicotine ratio, potassium and potassium-chlorine ratio. The amount of polyphenol was significantly correlated with K/Cl.The contents of nicotine and total nitrogen corresponding separately to three polyphenol samples of low, middle and high were not high different; the contents of total sugar, sugar-nicotine ratio, sugar-nitrogen ratio of these three samples separately had middle, low, high trend.4.The relationship of polyphenolic substances and organic acid indicates that neochlorogenic acid was significantly correlated with palmitic, oleic acid and linoleic acid, stearic acid, malic acid, acetylpropionic acid and total organic acids. Chlorogenic acid was significantly correlated with malonic acid, lactic acid, oxalic acid, citric acid. Quinic acid was significantly correlated with malic acid, acid and linoleic, total organic acid, hexadecanoic acid, stearic acid. Scopoline was significantly correlated with lactic acid, hexadecanoic acid, acid and linoleic, oxalic acid, citric acid, malic acid, total organic acid, propane diacid. Scopoletin was significantly correlated with lactic acid. Rutin was significantly correlated with lactic acid and levulinate. Kaempferol -3-rutinoside was significantly correlated with citric acid, malic acid, stesric acid, total organic acid,lactic acid and levulinate. Total polyphenols was significantly correlated with propane diacid, lactic acid and levulinate.The low polyphenol sample corresponding to the highest amount of lactic acid, levulinate, axalic acid, and the lowest amount of propane diacid, medium amount of total organic acid. The middle polyphenol sample corresponding to the highest amount of malic acid, medium amount of levulinate, propane diacid and citric acid, the lowest amount of total organic acid. The high polyphenol sample corresponding to the highest amount of propane diacid and total organic sugar, the lowest amount of lactic acid, levulinate, malic acid,citric acid. The amount of hexadecanoic acid, stearic acid, acid and linoleic had no significant chang between the three samples.The amount of lactic acid had significant diversity among these three samples. The amount of malic acid, citric acid, hexadecanoic acid, acid and linoleic corresponding to the three samples had no significant differences. The amount of propane diacid,stearic acid corresponding to the high polyphenol sample was significantly higher than the middle sample but no significant differences with the low sample. The amount of acetylpropionic acid corresponding to the middle polyphenol sample was significantly higher than the high polyphenol sample but no significant differences with low sample. The amount of stearic acid corresponding to the high polyphenol sample was significantly lower than the middle sample but no significant differences with middle sample. The amount of total organic acid corresponding to the high polyphenol sample was significantly lower than the middle sample but no significant differences with the low sample.5.The analysis of relationship between polyphenolic substances and neutral aroma components indicated that neochlorogenic acid and geranyl actone were highly significantly negative correlated; Chlorogenic acid were highly significantly negative correlated with megastigmatrie-nones. Quinic acid were significantly negative correlated with solanone andβ-damascone; Scopoline were significantly correlated with benzyl nalcohol, furfural, solanone, keto-solanone, geranyl aceone. Scopoletin were significantly correlated with furfural, benzaldehyde, benzyl alchohol, nyacinthin, benzyl ethanol,β-damascone, megastingmatrienone, total neutral aroma,furfural, dihydroactinidiolide. Rutin were significantly correlated with solanone, keto-solanone, benzyl alcohol, hyacinthin,β-damascone and megastigmatrie-nones; Kampferol-3-rutinoside were significantly correlated with benzyl alcohol,β-damascone, total neutral aroma, furfural, hyacinthin, phenylethanol,β-damascone, megstigmatrie-nones. Total polyphenols were significantly correlated with benzaldehyde and megastigmatrie-nones.Maillard reaction products, the degradation products from catrotenoids and total neutral aroma had the opposite changing regularity with polyphenolic substances between different samples, but the cembratriendid compounds had the same changing regularity with polyphenolic substances between different samples, that is to say, the amount of polyphenolic substances is high, middle, low samples of three groups, corresponding to furfural and furfural alcohol also show high, medium and low trend. The degradation products of aromatic amino acid had a different changing regularity with the changing trend of polyphenolic substances samples, high polyphenol sample corresponding to the lowest amount of maillard reaction products, the amount of maillard reaction products corresponding to the medium and low samples had little differences.Furfural, furfural alcohol, benzyl alcohol, benzyl ethanol,β-damascenone,β-damascone, geranyl acetone, dihydroactinidiolide, megastigmatrienone were consistent with the classification results of polyphenolic substances. Solanone had the different changing regularity with polyphenolic substances samples, solanone, keto-solanone corresponding to the low polyphenol sample had the highest amount, the amount of solanone, keto-solanone corresponding to the medium and low sample had no significant differences.The amount of Maillard reaction products and neutral aroma components had a significant difference between high and low polyphenol sample but had no significant difference with middle sample. The amount of degradation products from carotenoids had no significant differences between middle and low polyphenol sample but had significant differenc between high sample. The degradation products fo aromatic amino acid and the degradation products of cembratriendid compounds had no significant difference among three samples. The amount of furfural, benzyl alcohol, hyacinthin had no significant difference between high and low sample but had a significant difference with high sample. The amount of hyacinthin,β-damascenone, geranyl acetone had a significant difference between high and low sample but had no significant difference with middle sample. The amount of megastigmatrie-nones had no significant difference between middle and low sample but had no significant difference with high polyphenol sample.6.The initial evaluation of flue-cured tobacco aroma index indicated:the A value of different tobacco production area were: Dongbei > Fujian > Guizhou > Yunnan > Henan, Shandong > Brazil > Zimbabwe, which mean the effect of emitting aroma was best in Dongbei tobacco leaves and worst in Zimbabwe. The B value were Zimbabwe > Brazil > Fujian > Guizhou > Dongbei > Shandong, Henan > Yunnan, which mean Zimbabwe tobacco had the best aroma, followed by Brazil; The aroma index were Zimbabwe > Brazil > Fujian > Guizhou > Dongbei > Shandong, Henan > Yunnan, which mean Zimbabwe had the best smoking quality and aroma quality, Brazil followed.The total polyphenols were highly significantly negatively correlated with B value and aroma index; The tannin compounds were not significantly correlated with aroma index; Coumarins were highly significantly with B value and aroma index, and significantly correlated with A value; Brass alcohols were negatively and significantly correlated with B value and aroma index, were significantly correlate A value.
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