Studies On Key Factors Of Industrialized Production For Pleurotus Eryngii

Pleurotus eryngii is one of high quality edible mushroom. Recently, they have attracted considerable attention because consumer prizes its excellent testure, the nutritional and medical value. The result from the present investigations suggested that Pleurotus eryngii showed to modulate the immune system and have antioxidant capacity, in addition, supplementation of Pleurotus eryngii could also prevent the development of atherosclerosis.In this research, the nutritional physiology of Pleurotus eryngii was studied, the optimal condition of inoculum amount and volume of cultivation material on growth of mycelial growth of Pleurotus eryngii, the optimal temperature and the optimal moisture content of cultivation material also determined, the effects of cultivation materials and their interactions, the effects of spend mushroom substrate to the yield of Pleurotus eryngii were studied at last. The main research output was following:1. For the purpose of Pleurotus eryngii nutritional physiology, the size of colony and surface mycelium growth vigor were used to assess the effects of various carbon and nitrogen sources, their concentrations, minera elements, vitamins and phytohomones on growth of Pleurotus eryngii. The results show that fructose, glucose and mannose were the top three best carbon sources for mycelial growth. Fructose was the most utilized, followed in order by glucose and mannose. All the organic and inorganic sources investigated improved growth with peptone being the best, followed by malt flour and yeast extract paste. 1.0 g/L magnesium sulfate and monopotassium phosphate, 6 mg/L vitamin B2 and nicotince acid promoted good mycelial growth. Among the phytohormones tested, 8 mg/L IAA and KT stimulated mycelial growth.2. Seed dressing was used to probe the effects of inoculum amount and volume of cultivation material on growth of mycelial of Pleurotus eryngii. The optimal temperature and the optimal moisture content of cultivation material were also determined. The results indicated that the optimal temperature of mycelial growth of Pleurotus eryngii was 23℃and the optimal moisture content of cultivation material was 64.33 %. The optimal growth of mycelial was occurred when inoculum amount and volume of cultivation material set at 14.3 % and 1 000 g/bag, under optimal conditions, the complete colonization of the substrate needs 7 days.3. Experiments were performed to determine effects of supplementation of cottonseed hull with wheat bran, saw dust and superphosphate on production of Pleurotus eryngii using mixture design and quadratic regression orthogonal design, quadratic polynomial equation was developed to show the effects of each cultivation materials composition and their interactions for production of Pleurotus eryngii using response surface methodology (RSM). The model estimated that, a maximal biological efficiency (81.31 %) could be obtained when the concentration of wheat bran, saw dust and conttonseed hull were set at 21.63%, 6.27% and 72.10%, respectively.4. Effect of spent Pleurotus eryngii substrates, wheat bran, cottonseed hull and saw dust to the yield of Pleurotus eryngii were studied using proportional mixture design and ternary quadratic regression orthogonal methods. The corresponding mathematical model was established. The binary polynomial equation was established by response surface optimization method. The canonical analysis revealed that a maximal biological efficiency of 88.16 % could be achieved at the concentration point where the substrate contains 46.68 %, 12.83 %, 5.29 % and 35.20 % for spent Pleurotus eryngii substrates, wheat bran, saw dust, and cottonseed hull, respectively.