Study On Extraction And Separation Technology Of Resveratrol And Anthraquinone From Polygonum Cuspidatum Sieb.et Zucc.

Polygonum cuspidatum Sied.et Zucc.which belongs to Polygonaceae is a perennial herb plant. Resveratrol and anthraquinone are the most important active ingredients in Polygonum cuspidatum which has a variety of medicinal value. Adopting Polygonum cuspidatum from Hanzhong as the raw materials, study on coextraction, coseparation and copurification of resveratrol and anthraquinone from Polygonum cuspidatum , which provides a theoretical basis for industrial production.Study on the extracting time of polydatin and resveratrol from polygonum cuspidatum by using Soxhlet extraction method. The optimum extraction time has been identified. After Soxhlet extracting, the content of polydatin and resveratrol in polygonum cuspidatum Sied.et Zucc.is assayed by high performance liquid chromatography. HPLC on Symmertry-C18 column with acetonitrile-water (19.5:80.5) as mobile phase at 303 nm. The flow rate was 1.0 mL/min. Temperature of Column was 28℃. The result showed that the accumulation of polydatin and resveratrol increases with the extension of the growth years. The content of anthraquinone in polygonum cuspidatum Sied.et Zucc.is assayed by spectrophotometry. The result showed that the accumulation of anthraquinone also increase with the extension of the growth years. The content of anthraquinones in wild polygonum cuspidatum was higher than cultivated.For subsequent extraction, polydatin should be converted resveratrol. Study on zymolytic condition by orthogonal test. The optimum condition was temperature 45℃, solid-liquid ratio 1∶3 and fermentation time 48h. Conversion rate of polydatin was 96.1% after fermentation. Researching extracted condition by orthogonal experiment. The optimum conditions of extraction were 95% ethanol, solid-liquid ratio 1∶6, extracted 3 times and each time 1h. As a result, the content of resveratrol was 1.912% and the content of anthraquinone was 1.504%. Concentrating and vacuum drying extract into crudeⅠ, the yield of crudeⅠwas 11.11%; the content of resveratrol was 15.31% in crudeⅠ, and the content of anthraquinone was 11.83% in crudeⅠ.According to chemical property of resveratrol and anthraquinone, selecting mixed solvent(F∶B=1∶3) to convert crudeⅠinto crudeⅡ. The content of resveratrol was 33.1% in crudeⅡ. The content of anthraquinone was 25.98% in crudeⅡ. The extraction rate of resveratrol was 90%, and the extraction rate of anthraquinone was 91.41%.Adopting 50% ethanol to dissolve crudeⅡ, resveratrol was dissolved in the solution and anthraquinone was precipitated in the deposition. Drying deposition into primary product of anthraquinone. The content of anthraquinone was 50.82% in primary product.By static adsorption experiments, selecting the best macroporous resin D101 for purifying anthraquinone. After purifying primary product of anthraquinone, the content of anthraquinone was 71.51% in final product. Using macroporous resin A to purify resveratrol solution, the content of resveratrol was 97.78% in final product.Using dry and fresh polygonum cuspidatum as raw materials, verifying complete technics by natural fermenting, reflux extracting, dividing impurity, separating and purifying. In the resveratrol product of dry polygonum cuspidatum , the yield of resveratrol was 1.26%, the content of resveratrol was 97.87%. In the anthraquinone product of dry polygonum cuspidatum, the yield of anthraquinone was 1.34% , the content of anthraquinone was 71.71%. In the resveratrol product of fresh polygonum cuspidatum, the yield of resveratrol was 0.6%, the content of resveratrol was 98.01%. In the resveratrol product of fresh polygonum cuspidatum Sied.et Zucc., the yield of anthraquinone was 0.52% , the content of anthraquinone was 70.83%.