Font Size: a A A

Study On Mating Circadian Rhythm And Sex Pheromone Crude Extracts Of Stomphastis Thraustica Meyrick

Posted on:2011-08-30Degree:MasterType:Thesis
Country:ChinaCandidate:N JiangFull Text:PDF
GTID:2143360308972174Subject:Agricultural Entomology and Pest Control
Abstract/Summary:PDF Full Text Request
Stomphastis thraustica Meyrick is an important leaf miner of Jatropha curcas L. The emergence behavior, emergence rhythm, adults' longevity, ratio of sex, mating behavior, mating rhythm, morphological characteristics of antenna sensilla, males' response to EAG and attractiveness of brown sugar, wine, vinegar were studied in this paper. The results were as follows:1. The population of Stomphastis thraustica was set up in laboratory and its emergence behavior, emergence rhythm, adults' longevity, ratio of sex were also observed in laboratory. The emergence behavior of Stomphastis thraustica was similar to other Lepidoptera insects. They all experienced a series of behavior like breaking pupae, getting out of cocoon, spreading wings. The emergence number in dark period was more than in photophase. During 20:00~23:00, the emergence rate of adult was 66.35%. No mating adults' average longevity was longer than the mated ones. Female moths'average longevity was longer than males. The ratio of sex (♀/♂) of Stomphastis thraustica was 0.87 and the number of male moths was little more than females.2. Scanning electron microscopy was used to observe antennae and the sensilla of Stomphastis thraustica. Five types of sensilla including sensilla trichodea, sensilla chaetica, sensilla coeloclnica, sensilla basiconca and sensilla squamiformia were found on antennae. The morphological characters of these sensillas of Stomphastis thraustica were described respectively. The number of sensilla trichodea was the largest in the five types of sensilla. In addition, the sensilla trichodea consisted of longer and shorter sensilla trichodea. The sensilla chaetica was also consisted of longer and shorter sensilla chaetica.3. Adults' mating behavior and circadian rhythm of Stomphastis thraustica were investigated at (25±1)℃and under 16L:8D. The courtship and mating behavior of Stomphastis thraustica was similar to other Lepidoptera insects. No matter female or male,1-day-old adults could mate, the highest mating frequency reached 70.12% when the adults were all 1-day-old. With adults' age growing, the mating frequency decreased, the lowest mating frequency was only 5.81%, but the mating time increased from 89.33 min to 171.67 min. Adults had the highest mating frequency in the first hour of photophase. Under different conditions of the illumination intensity, all adults could mate. With illumination strengthening, the mating frequency of 1-day-old increased from 53.33% to 69.40%. But different illumination intensity had little influence on mating time of 4 and 5-day-old adults.4. The results of males' EAG response to sex pheromones showed that the extracts which were extracted from virgin female sex pheromone glands by hexane were more active than that obtained by other solvents. The value of EAG response was 2.22mv. With male adults' age growing, the value of EAG response to sex pheromones decreased from 2.37mv of 1-day-old adults to 0.38mv of 4-day-old adults. From 1-day-old adults to 4-day-old adults, the values of EAG response of extracts obtained by solvent were all higher than by air collection. The dosage curve of sex pheromones showed that the lowest concentration of males' EAG response to sex pheromones was 10-2FE/10μl.5. Olfaction taxis of Stomphastis thraustica to different volume ratio of brown sugar, wine, vinegar was analyzed by using 'Y' pipe and luring Stomphastis thraustica of different volume ratio of brown sugar, wine, vinegar was studied in the field. The results were as follows:When the volume ratio of brown sugar, wine, vinegar, water was 1:1:2:20, Stomphastis thraustica had the best olfaction taxis and the average rate of luring moths was 66.67%. The result of the test in the field showed that this mixture had the best effect and the rate of luring moths in the field was 6.67%. Therefore, the best suitable mixture luring Stomphastis thraustica was that the volume ratio of sugar, wine, vinegar, water was 1:1:2:20.
Keywords/Search Tags:Stomphastis thraustica, sex pheromone, mating circadian rhythm, SEM, EAG
PDF Full Text Request
Related items