The Experimental Study Of Function Of Yi-xinkang On CVB_3m Viral Myocarditis In Mice

Object: The experimental study selected the animal model of experimental viral myocarditis in Blab/c mice in order to study the effects of Chinese medicine Yi-xinkang in general state on these mice, in myocardial protection and in inhibition of viral replication in myocardial cells. Mechanism of function that the medicine treated viral myocarditis was preliminarily understood so that the new medicine could be looked for to treat viral myocarditis and a kind of effective way could be offered to clinical practice. Method: 75 male Balb/c purebred mice ,5 to 6-week-old , weigh49?g , were randomly divided into 5 groups : Yi-xinkang group (YI), Ribavirin group(RI), normal saline group(NS) , normal saline control group(NSC) and blank control group(BC) . Every group had 15 mice. The mice of YI, RI and NS were infected by coxsackie virus B3m (CVB3m) through abdominal cavity injection to develop animal models of experimental myocarditis in Balb/c mice, and were treated respectively by Yi-xinkang, Ribavirin and normal saline for a week. The mice of NSC were infected by MEM Eagle liquid without virus and treated by normal saline for a week. The blank control group wasn't infected and treated. The other feeding conditions were same. Ordinary conditions of mice in every group were dynamically observed including their weight. CK and CK-MB of serum in each mouse were measured through Beckman automatic biochemistry analytical instrument after the mice were infected a week. 7 infected mice were randomly collected from every infected group, and amount of CVB3m RNA was measured from their myocardium. Result: After the mice were infected 3 days, general state of the infected groups was obviously worse than the non-infected groups, and general state of YI group was the best in three infected groups. Meanwhile, average weight of the infected groups was decreased, and average weight of YI was the highest in three infected groups. After the mice were infected 7 days, CK and CK-MB of serum in the infected groups were more increased than in non-infected groups, and those in YI were the lowest in three infected groups. Meanwhile, average of CVBsm RNA in the myocardial cells of YI was the lowest in the three groups. Conclusion: The results of the experiment suggest that Yi-xinkang might play an important role in protection of CVBsm viral myocarditis in the Balb/c mice, while it might reduce harm of viral myocardial cells in the infected mice and inhibit CVB3m replication in myocardial cells.