Expressional Significance And Correlative Analysis Of EGFR And PCNA In Dysplasia Of Oral Mucosal Epithelium And Squamous Cell Carcinoma

Using S-P immunohistochemical method to study the significance of expression and relationship between epidermal growth factor receptor(EGFR) and proliferating cell nuclear antigen(PCNA) in dysplasia of oral mucosal epithelium and oral squamous cell carcinoma(OSCC).To study the effect of EGFR and PCNA in the initiation and progression of OSCC and to evaluate the relationship between EGFR,PCNA and histological classification of OSCC ,metastasis of lymph and prognosis of the patients. The results showed that EGFR and PCNA immunostaining were negative in normal epithelium and there were only a few positive cells limited to the basal layer. In dysplasia, as the lesions were progressing, the positive expression accordingly increased(P < 0.01). The expression of PCNA had no significant difference between severe dysplasia and carcinoma(P > 0.05). The rate of EGFR expression in severe dysplasia was higher than that in well differentiated carcinoma. As the differentiated degree of carcinoma was decreasing , the positive rate of EGFR and PCNA increased in OSCC(P < 0.01). Statistically positive expression of EGFR was correlated with metastasis of regional lymph and prognosis of the patients(P < 0.0005). The OSCC with lymph metastasis showed a higher PCNA expression than that without lymph metastasis(P < 0.0005). The patient of OSCC with high PCNA expression had a shorter survival period than that with low PCNA expression(P=0.013). There was a closecorrelation between EGFR and PCNA in expression(P < 0.005). The expression ofEGFR and PCNA is related to the initiation and progression of OSCC. EGFR andPCNA may be used as a useful marker for assessing proliferative status of carcinomacells, predicting the malignant change of oral mucosa, determing the malignant degressof carcinoma and the tendency of regional lymph metastasis and prognosis of thepatients.