| Cancer cells have increased rates of glucose metabolism when compared to normal cells, one of the mechanisms proposed for the accelerated glucose use in malignant cells is the overexpression of glucose transporters (GLUT). GLUTi is a facilitative cell surface glucose transporter protein which is responsible for the basal glucose uptake of cells. A number of immunohistochemical studies have demonstrated that GLUTi is undetectable in normal epithelial tissues and benign epithelial tumors but is aberrantly expressed in a wide spectrum of epithelial malignancies. The aberrant expression is closely related to neoplastic progression. However, to this day little information was available on GLUTi expression and its regulation in ovarian cancer. This experiment immunohistochemically detected GLUTi expression in normal, benign tumors, borderline tumors and epithelial ovarian carcinomas. Furthermore, the relationship between GLUTi expression and the expression of bFGF (basic fibroblast growth factor) and PCNA (proliferating cell nuclear antigen) were analysed to investigate the function of bFGF and GLUTi in the pathogenesis and development of ovarian epithelial carcinomas (OEC), which offer an effective theory basis for early diagnosis, therapy, prognostic prediction of ovarian epithelial carcinoma.MethodsStreptavidin-Peroxidase complex technique was used to examine the expression ofGLUTi, bFGF and PCNA protein in 6 cases of ovarian normal tissue, 20 cases of benign epithelial tumors, 7 cases of borderline tumor and 44 cases of epithelial ovarian carcinoma (including 25 mucinous type, 12 serous type, 7 endometrioid type. Of the ovarian cancer patients, 29 accompanied with tumor diffusion, 20 accompanied with lymph node metastasis. According to the histological grading, The 44 cases of ovarian carcinoma were divided into three groups: 10 cases of GI, 14 cases of GI and 20 cases of GS). Furthermore, their correlations with the clinicopathologic features and the relationship between GLUTi expression and the expression of bFGF and PCNA proteins were investigated.Results1. In normal ovary, benign tumor, borderline tumor and ovarian cancer, the positive expression rate of GLUTi was 0, 0, 85.7%(6/7), 91%(40/44) respectively; The positive rate of bFGF was 0, 10%(2/20), 57.14%(4/7), 56.82%(25/44) respectively; PCNA mean score was 0.0517, 0.0586, 0.3100, 0.5423 respectively. The expression rates and the mean score in OEC tissues were significantly higher than those in benign tumor and normal ovarian tissues. The differences were significant (P<0.05). The intensity of GLUTi in OEC (1+ n=4, 2+ n=ll, 3+ n=25) was significantly higher than borderline tumors (1+ n=4, 2+ n=2). The difference was significant.2. The GLUTi staining showed an intense staining of the cytoplasmic membrane, the reaction intensity varied from cell to cell and was stronger in areas farther away from blood vessels and near the necrotic center.3. The intensity of GLUTi staining and PCNA score were significantly associated with the histological grade of the tumor. With the increase of histological grade, theintensity of GLUT, staining and the score of PCNA increased (rs =0.499 P=0.001, P<0.05).bFGF positive rate in tumor of Gi,G2,G3 was 20%, 57.14%, 75%. The rate of G2 and G3 have no significant difference but significantly higher than GI (P<0.05).4. The staining intensity of GLUTi in the advanced stage (III-IV) was higher than that in the early stage ( I -II) (P=0.001). The positive rate of bFGF in early stage and advanced stage of ovarian cancer was 33.33% and 73.08%, the difference was significant(x2=6.848 P=0.009). The mean score of PCNA in advanced stage and early stage was0.6292 and 0.4167, the difference was significant (P=0.001).5. The staining intensity of GLUTi, the positive rate of bFGF and PCNA score increased with ovarian cancer diffusion and lymph node metastasis (P<0.01).6. GLUTi expression level did not show any statistical association with histology type(P=0.513).7. The staining intensit...
|
PDF Full Text Request