The Expression And Effect Of CHAF1A And PCNA In Cervical Squamous Cell Carcinoma

PART ONE THE EXPRESSION OF CHAF1 AAND PCNA IN CERVICAL CANCERObjective To investigate the expressions of chromatin assembly factor 1 subunit A(CHAF1A)and proliferating cell nuclear antigen(PCNA)in cervical squamous cell carcinoma(CSCC)and their clinical significance.Methods Real-time quantitative PCR was performed to detect the m RNA levels of CHAF1 A and PCNA in cervical squamous cell carcinoma and corresponding paracancerous tissues.Immunohistochemistry was used to detect the expressions of CHAF1 A and PCNA protein in normal cervical squamous cell epithelium tissues(NC),cervical intraepithelial neoplasia tissues(CIN),and CSCC.The correlation between CHAF1 A and PCNA expression and the relationship of the two proteins to the clinical pathological features of cervical cancer were analyzed.Results The expressions of CHAF1 A and PCNA in CIN and CSCC were significantly higher than that in NC.In addition,CHAF1 A and PCNA expressions were positively correlated to CSCC tissues.Furthermore,overexpression of CHAF1 A was significantly associated with the degree of differentiation,tumor size,depth of cancer invasion and HPV infection.Finally,the up-regulated expression of PCNA was correlated with the degree of differentiation,International Federation of Gynecology and Obstetrics(FIGO)grade,lymph node metastasis,depth of cancer invasion and HPV infection.Conclusion CHAF1 A and PCNA are highly expressed in CSCC and associated with malignancy.PART TWO EFFECT OF CHAF1 A ON BIOLOGICAL BEHAVIOR OF CERVICAL CANCER CELLObjective To investigate the effect of CHAF1 A gene expression on the proliferation,migration and apoptosis of cervical squamous carcinoma cells.Methods The expression of CHAF1 A was detected by q RT-PCR and Western blot in different cervical cancer cell lines and normal human immortalized epidermal cell lines.The endogenous expression of CHAF1 A was interfered,cell proliferation was detected by MTT assay,cell apoptosis and cell cycle was detected by flow cytometry,changes in cell migration ability were detected by scratch test,and proliferation-related apoptosis factors and cell cycle-related factors were detected by western blot.Results CHAF1 A was highly expressed in cervical cancer cells.The proliferation and migration of sh CHAF1 A group cells were lower than NC group,and the proportion of apoptosis increased.These results also suggested inhibition of G1 to S phase transition.Conclusion The decreased expression of CHAF1 A inhibits cell proliferation and migration and promotes cell apoptosis.