The Expression Of PTEN,EGFR,P16 In Astrocytoma And Their Significance By Tissue Microarray

[Objective] : To study the expression of PTEN, EGFR and P16 in astrocytoma ,combining the expression of 53, Ki-67 LI, histological grading and tumor size, to make clear the relationship between the biomarks and astrocytoma,and to provide the valuable information to gene diagnose and prognosis in astrocytoma.[Methods] : Building a tissue microarray including 224 spots and using immunhistochemical method, the expression of PTEN, EGFR, P16, P53 and KI-67 was investigated in 64 astrocytoma diseases and 10 normal brain tissues.[ Results ]: 1,The expression of PTEN, P16 was more lower in astrocytoma diseases (grade â…¡, â…¢, â…£) than in normal brain tissue and grade I ,EGFR was statistically significantly more higher; 2, PTEN, P16 showed negativecorrelation to astrocytoma grading, Ki-67 and P53,On the contrary ,EGFR was statistically positively related to these markers; 3, PTEN and EGFR showed negative correlation relationship, and both of them showed no statistic relation to P16.[Conclusion] :1, PTEN, EGFR and P16 involve in the development of astrocytoma ,and influence the biological behaves of astrocytoma . 2, The expression of PTEN, EGFR and P16 was possibly used to evaluate the prognosis of astrocytoma . 3, There were more than three independent gene pathways (including PTENgene , EGFRgene and P16gene) in the development and progression of astrocytoma ; and what' s more , the pathogenesis of grade I were different from grade â…¡, â…¢, â…£ immunohistochemical positivity for PTEN and P16 in patients were associated with a longer survival time; However EGFR protein positivity was related to a shorter survival time. 4, To study the expression of PTEN,EGFR and P16 protein will build up target gene therapy foundation of astrocytoma . 5, Tissue Microarray (TMA) is an effective high-throughput technique platform for the study of tumor molecular pathology.