The Study About Relationship Between Different Dose Of Alcohol Intake And Avascular Necrosis Of The Femoral Head

Purpose: To study the relationship between different dose of alcohol intake and femoral head necrosis in rats, in order to establish an ideal and practical animal model which might investigate the pathogenesis of femoral head necrosis.Method: 30 rats were randomly divided into 3 groups, each group contents 10 rats, experimental group I(EG I) were administered the wine containing 56% of alcohol at dosage of 8ml/(kg d), experimental group II(EG II) were administered the wine containing 56% of alcohol at dosage of 6ml/(kg d), and control group conduct same amount of NS respectively. All animals were killed in 20 weeks after the start of the procedure. Detective index as followed: the changes of histology in the femoral head, the items of lipid metabolism, hemorheology, oxygenfree radical(OFR), endothelin(ET) and nitric oxide(NO). Results: (1)The conditions of rats in control group were better than rats in other groups. In experimental groups(EG I and EG II), the weight increased slowly and the cartilage of the femoral head were destroyed. Pathological changes of EG I was more obvious than those of EG II. (2)In experimental groups, empty osteocyte lacuna increased and trabecula of bone decreased, ruptured. Pathological changes of EG I was more obvious than those of EG II. (3) Quantities of empty osteocyte lacuna: EG I>EG II>Control. there were significant difference while each group was compared with another(p<0.05 or p<0.01). (4)Electron microscope analysis showed osteocytes and osteoblasts in experimental groups decreased and osteocytes had degeneration signs or fatty drips appeared inside osteocytes, and intramedullary lipocytes became larger in experimental groups. Pathological changes of EG I was more obvious than those of EG II. (5)Levels of TC, TG, LDL: EG I>EG II>Control, there were significant difference while each group was compared with another(p<0.05 or p<0.01). Levels of HDL: EG KEG IIEG II>Control, there were significant difference while each group was compared with another(p<0.05 or p<0.01). RBC aggregation index and whole blood relative viscosity at high-shear rate: EG I>EG II>Control, there were significant difference between EG I and control(p<0.05 or p<0.01). (7) Levels of MDA: EG I>G II>ontrol, there were significant difference between EG I and control (p<0.05). Levels of SOD: EG KEG IKControl, there were significant difference between EG I and control(p<0.05). (8)The level of plasma ET: EG I>G II>ontrol, there were significant difference while each group was compared with another(p<0.05 or p<0.01). (9)The level of serum NO: EG KEG IKControl, there were significant difference between EG I and control(p<0.05).Conclusion: (1)the result suggested that intake of high dose of alcohol would induce the femoral head necrosis, and the positive correlation was found between the dosage of alcoholintake and degree of osteonecrosis. (2)Disorders of lipid metabolism and hemorheology induced by intake of high dose of alcohol are the most important pathogenesis of the femoral head necrosis. (3)Disorders of OFR metabolism are involved in pathology procedures of the femoral head necrosis. (4)Increasing of the level of plasma ET have important function in the pathology procedures of the femoral head necrosis. Decreasing of the level of serum NO are involved in pathology procedures of the femoral head necrosis.