The Effect Of Compound Compnents Of Sanchi In Rhesus Monkey's Endomitrial Histomorphology With Abnormal Uterine Bleeding Caused By Endometritis

Objective: By studying the changes of rhesus monkey's endomitrial histomorphology with abnormal uterine bleeding caused by endomitritis and the therapy effect of compound utility components of sanchi, discuss the mechanism of action in Huayuzhixue of sanchi, and give help for clinical therapy of inflammatory menorrhagia.Methods: 9 cases of female rhesus monkey with normal menses were divided into three groups, including Chinese medicine group(3 cases), model group(3 cases) and normal control group(3 cases). Injected the mixed bacteria to the two group(mcluding Chinese medicine group and the model group) rhesus monkey's uterus to induce endomitritis. The mixed bacteria was colibacillus, staphylococcus aureus and streptobacillus. It's proportion was 1:1:3. Five day's later, bacteria cultured to indicate there was bacteria growing in the uterus. Then observed the menstruation change. Bred with compound utility components of sanchi to the Chinese medicine group two days before the next menstrual cycle for seven days. After three menstrual cycles' therapy, cut all rhesus monkeys' uterus within 24 hours prior to their fourth menstrual cycle. Observed the changes of uterine histomorphology under light and electron microscope.Result: (1) Contrasted with normal control group, model group was menorrhagia and the menstrual period was long, Chinese medicine group was better than the model group. (2)Under electron microscope model group's endomitrium was found not only in flammatory cells infiltrate and endothelial cells degenerate and edema, but glandular epithelium cytoplasm edema was well as endothelial spiral uterioles cell of edema and vacuolate, Chinese medicine group's endomitrium was found only few inflammatory cells infiltrate and few endothelial cells degenerate and edema.(3)Under light microscope, model group's endomitrium was found not only inflammatory cells infiltrate and bleeding in the stroma, endometrium epithelium necrosis superficial micrangium and venous sinus dilatation, but spiral arteriolar lumen was obvious dilatation especially around the gland dilatated and filled with inflammatory exudates. the Chinese medicine group's endomitrium was found few inflammatory cell infiltrate and hyporrhe in the stroma, spiral arteriolar luman was subdilatation.Conclusions: All above suggested that inflammation cause the spiral arteriolar lumen deletation and degenerate which result in endomitrium bleeding. Compound components of sanchi can decompress in flammation, relieve degenerate and enhance the spiral arteriolar lumens' contraction to hemostasis.