| Objective: To ascertain the relationship between serum levels of Resistin and tumor necrosis factor-alpha (TNF- α ) in Type 2 diabetes mellitus patients and their association with IR.Methods: 30 non-obese DM subjects (16men, 14women, age48± 9) ,30 obese DMsubjects(15men, 15women,age50±8), and 30 normal subjects (14men, 16women, age46±10) were selected randomly. The serum Resistin and tumor necrosis factor-alpha ( TNF- α ) levels were measured by enzyme-linked immunosorbent assay, and their fast glucose (FPG) , total-serum cholesterol (TC) were measured at the same time. The body height, weight and blood pressure were measured, and then the BMI and WHR were calculated; the insulin resistance index were calculated by homeostasis model assessment (Homa) ; The results were compared between them.Results: The serum Resistin levels and serum TNF- α levels are much higher in the non-obese DM subjects and obese DM subjects than in the normal control group. The difference is significant(P<0. 05,P<0. 01). The difference of the serum Resistinlevels and serum TNF- α levels between the non-obese DM subjects and obese DM subjects were significant(P<0. 05). The serum resistin level showed positive correlation with BMI.FPG, HA1C, TG and IRI(r=0.40,P<0 .05;r=0.37,P<0. 05;r=0.38, P<0. 05;r=0.41,P<0. 05; r=0.49,P<0. 01). The serum resistin level had no association with insulin, TCH, SBP, DBP and WHR; The serum TNF- α levels positively correlated to BMI, FPG, HA1C , TG, FINS and IRI ( r=0.406,P<0.001 ; r=0.274,P<0.01;r=0.265,P<0.01 ;r=0.356,P<0.001 :r=0. 428P<0.001; r=0.49,P<0 01). It had no association with WHR, SBP. Multiple regression analysis showed the serum level of resistin and TNF- α positively correlated to IR.Conclusion: The insulin resistance is more significant in type 2 diabetes mellitus patients especially in obese type 2 diabetes mellitus patients. Resistin and TNF- α are probably the important factors affecting the insulin resistance. |
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