| ObjectiveRadiofrequency ablation ( RFA) is safe and effective for the treatment of liver tumor , and it is gaining increased attention clinically . However, it has its own limition , such as , its extention of coagulation necrosis with one RFA procedure is limit. Although a combined therapy of RFA and injection of hypertonic saline or acetic acid could increase the extention of induced coagulation necrosis , which effect is better and more safe ? and if their mixture has enhancement for RFA ? How is its side effect ? In this experiment , We wanted to compare the effects of hypertonic saline , acetic acid and acetic acid - hypertonic saline solution ( AAHS) - enhanced hepatic radiofrequency and generally evaluate the effects of these three solution . We also wished to dertermine the optimal solution in order to maximize its effect on extent of the RFA - induced coagulation necrosis .Material and MethodsThirty rabbits were assigned to one of six groups : group A : RFA alone ( n = 5 ) ; group B : RFA with 36% Nacl injection ( n = 5 ) ; group C : RFA with 50% acetic acid injection ( n = 5) ; group D : RFA with 50% AAHS ; group E : 50% acetic acid injection alone ; group F : 36% Nacl injection alone . On o-pening the rabbits epigastrium, A volume of 1 ml varies solution were injected into the liver respectively before RFA . LDRF - 120S multi - electrode Radiofre-quecy generator( Mian Yang Lide ltd. Sichuan) was used in all experiments .The RF energy (15 W) was applied until it stopped automatically . After operation or following - up , the rabbits were sacrificed and excised the liver , and fixed in 10% formalin . Then measured the long and transverse diameter , compared the areas of radiofrequency induced coagulation . Representative samples were processed with paraffin section and hematoxylin - eosin ( HE) staining for light microscopic study , and evaluate these three solution totally by the features of imaging and pathologic histology .ResultsDuring the radiofrequency , the tissue impedance in the rabbits of A,B,C, D group decreased gradually at first until a lowest value emerged . and then rised up quickly until the procedure finished . Compared the lowest impedance value for group A with B,C,D group' s, the difference were statistically significant (p <0.05) . Compared lowest impedance value for group B,D with group C p < 0. 05 . However , the difference in lowest impedance for group B and group D werent statistically significant (p =0.997) . There were differences a-mong radiofrequency enery applied persistent time of groups . Persistent time in group A was significantly shorter than in group B or D. (p<0.05) . However , the difference between in group B and in group D was not statistically significant (p >0.05) . Meantime , the differences between in group C and in group B or D were statistically significant (p <0. 05) . The groups which injected solution before RFA , Coagulation necrosis areas in group B,C,D were larger than in group A (p <0. 05) . The lesion area in group D was maximum , the differences were statistically significant (p<0.05) . Meantime , the differences between in group B and in group A,C,E were statistically significant (p <0.05) . In follow - up rabbits , there was an enhanced rim peripheral coagulation in enhanced CT scan one week after procedure . correlation with a lot of leukocyte in-filtrated on pathology . Three weeks after procedure , the former enhanced rim disappered , and the coagulation necrosis area diminuted , which correlated with formed fabric envelope on pathology . Procedure - related complications occurred in six of twelve (6/12 , 50% ) :chemical peritonitis (abdominal adher-...
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