Expression And Clinical Significance Of CD15,CD44v6 And PCNA In Breast Cancer And Breast Hyperplasia

Objective: To evaluate the value of CD 15 antigen, CD44v6 protein and PCNA in differential diagnosis and early diagnosis of breast cancer by determining the level of CD 15 and CD44v6 expression in breast cancer , breast hyperplasia and normal breast ; To analyze the relationship between the three markers and clinicopathologic parameters of breast cancer; To study the correlation between their expression in breast cancer .Methods: 118 cases of female patients were selected: 51 cases of the patients were pathologically diagnosed as breast infiltrating duct cancer with median age of 54 years(28~76years); 67 cases of the patients were pathologically diagnosed as breast hyperplasia with median age of 39 years (25~52years)。 In addition , 10 cases of accessory mammary gland tissues replaced normal breast tissues as control with median age of 33.5 years(23~30years) . The expression of CD 15 antigen and CD44v6 protein in 51 cases of breast cancer,67 cases of breast hyperplasia and 10 cases of accessory mammary gland tissues were investigated by S-P immunohistochemical technique。 Statistical methods: chi-square test and rank correlation analysis were used (SPSS statistical software,Version 10.0 for windows) 。Results: The positive expressive rates of CD15 antigen in 51 cases of breastcancer , 67 cases of breast hyperplasia and 10 cases of normal breast tissues were 78.4% , 44.8% and 20% respectively; The positive expressive rates of CD44v6 protein were 80.4%, 37.3% and 20% respectively; The positive expressive rates of PCNA were 88.2%, 68.7% and 30% respectively . There was significant difference between breast cancer and breast hyperplasia(P<0.05)。 With respect to CD 15 antigen and CD44v6 protein expression ,there was not significant difference between breast hyperplasia and normal breast(P>0. 05) ,while considering PCNA expression there was significant difference between breast hyperplasia and normal breast (P<0.05)。 The positive expressive rates of CD15 antigen , CD44v6 protein and PCNA in patients with breast cancer were significantly correlated with axillary lymph node status and histological grading(P<0.05), while no significant correlation with patients' age , menopause status and TNM staging (P>0. 05 )。 The positive expressive rates of CD15 antigen , CD44v6 protein and PCNA were 89.2%. 83.8% and 97.3% respectively for patients with axillary lymph node metastasis, significantly higher than those without metastasis (42.9%, 64.3%, 64.3% respectively); The positive expressive rates of CD15 antigen , CD44v6 protein and PCNA were 86.1%, 88.9% and 94.4% respectively for patients of histological grading Ⅱ — Ⅲ, significantly higher than those of histological grading Ⅰ (60%, 60% and 78.6% respectively) . Moreover, there was a positive correlation between CD 15 and CD44v6 expressions in breast cancer (r=0.43,P<0.001 ); positive correlation between CD 15 and PCNA expressions in breast cancer (r=0.48,P<0.001); positive correlation between CD44v6 and PCNA expressions in breast cancer(r=0.34,P<0.001).Conclusion: The expression of CD 15 antigen, CD44v6 protein and PCNA may be related to the carcinogenesis and progression of breast cancer ; Combining detection of CD 15 antigen . CD44v6 protein and PCNA may be useful in differential diagnosis between benign and malignant lesions of breast ; The expression of CD 15 antigen, CD44v6 protein and PCNA might be useful indicators to evaluate the malignancy and biological behaviors ,and could beconsidered as good metastatic and prognostic predictors for breast cancer.