Study On The Expression And Clinical Significance Of TGF-β₁, CD105 And CD34 In Pancreatic Carcinoma

Objective: To study the effects of the expression of TGF-β₁ CD 105 and CD34 on growth, invasion and metastasis in pancreatic carcinoma, in order to provide some valuable evidence for prognosis and biotherapy.Methods: The expression levels of TGF-β₁,, CD105 and CD34 were detected by S-P immunohistochemistry in 52 cases of pancreatic carcinoma and 10 cases of non-tumorous pancreas tissue. Their relationship with clinic-pathological features and survival was also analyzed.Results: (1)The expression ratios of TGF- β₁ in non-tumorous pancreas tissue and in pancreatic carcinoma were 30% and 80.77% respectively, having significant difference (P<0.01). The TGF- β₁ expression level positively correlated with clinical stages and metastasis of lymph nodes (P<0.01), while no significant difference was observed in sex, age, the location and the size of tumor and histologic grade. (2)The MVD marked by CD105 in 52 cases of pancreatic carcinoma was 10.14±3.07 and in non-tumorous pancreas tissue was 1.03±0.27(P<0.01). The lower differentiation of pancreatic carcinoma, the higher MVD was. The MVD in stage III-IV was evidently higher than in stage I—II (P<0.01), and the MVD was also higher in those with metastasis of lymph node than those withoutmetastasis (PO.01), but there was no remarkable difference in sex, age, the location and size of tumor. (3)The MVD marked by CD34 in pancreatic carcinoma(34.53±8.29) was observably higher than that in non-tumorous pancreas tissue(16.78±4.92)(/><0.01). The MVD in stage IIMV was much higher than that in stage I—II (P<0.01). In the same way it was higher in those with metastasis of lymph node than without metastasis (P<0.0\), finding no significant difference in sex, age, the location and size of tumor and histologic grade. (4) There were statistically significant correlation between TGF- 0 , and CD105, TGF- 0 , and CD34. CD105 had a fine specificity in pancreatic carcinoma contrasting to CD34. (5)The analysis of single factor revealed that the expression of TGF- 0 ,, CD 105 and CD34 was significantly related with the prognosis of patients (PO.01).Conclusion: TGF- 0 i plays an important role in growth, invasion and metastasis in pancreatic carcinoma. Although CD 105 and CD34 are both markers of endothelial cells, CD 105 is more accurate to reflect the proliferation of them.