Expression And Significance Of S100A4,E-cadherin And P27 In The Non-small Cell Lung Cancer

Lung cancer, one of the most frequent malignant tumor world-wide, is well-known for its higher invasive, rapid growth, metastatic activity and poor prognosis. The emergence, development and metastasis of lung cancer is a complex process. S100A4, as a member of the S100 calcium-binding protein family, many investigators have demonstrated that S100A4 may make wild-type p53 mutate, prevent from cell apoptosis and cause tumor occurrence; otherwise, its overexpression is strongly correlated with tumor invasive and metastatic behavior. Transfection of the S100A4 gene into non-metastatic tumor cells induces metastatic capability, whereas antisense S100A4 RNA or anti-S100A4 ribozyme suppresses metastatic capability of high-metastic tumor cells. S100A4 play an important role in the emergence, devolepment and metastasis of malignant tumor. E-cadherin is a member of the cadherin superfamily that mediates calcium-dependent cell-to-cell adhesion. Dysfunction of E-cadherin leads to various malignant phenotypes such as cell morphologic change, loose cell-to-cell contact, and enhanced cell motility, so that E-cadherin is a significant factor in the suppression of cancer metastasis. As a tumor suppressor, p27 may regulate negatively the cell division cycle, control the G₁-S checkpoint; Overexpression of p27 protein in tumor cells induces a G₁ block of the cell cycle, reversely subexpression of protein causetumor occurrence . This study was designed to investigate the expression and relationship among S100A4n E-cadherin and p27 protein expression in non-small lung cancer (NSCLC) and to explore the correlation of S100A4> E-cadherin and p27 protein expression with clinicopathologic features of NSCLC. In order to offer a new idea to diagnosis, prevent and treat primary lung carcinoma.Methods: Eighty-six non-small cell lung cancer specimens and twenty normal lung tissue specimens were obtained from patients who underwent a surgical resection in the departments of Thoracic Surgery of the First Affiliated Hospital from 2001 to 2002 and of the Second Affiliated Hospital in 2003. The pathological stage was classified according to the UICC TNM classification, and the histological typing and differential grading of lung tumors were assigned according to the WHO criteria. The SP immunohistochenical method was used to detect the expression of S100A4^ E-cadherin and p27 in 86 cases of non-small cell lung cancer (NSCLC) and 20 case s of normal lung tissues. Statistical analysis was executed by SSPS 10.0 Software, with a =0. 05 regarded as statistically significant. Result: 1. The positive expression rates of S100A4 was observed in 55. 8% (48 of 86) NSCLCs; The reduced expression of E-cadherin and p27 was observed in 39. 5%(34 of 86) and 34.9%(30 of 86) respectively. In normal lung there was no the positive expression of S100A4, but the positive expression rate of E-cadherin and p27 were 95% and 100% respectively; There were significant differences between NSCLC and normal group (K0. 05).2. The expression of S100A4 was positively correlating with the size of age(K0.05K tumor size(R0. 005) ^ lymph node metastasis (K0. 005), TNM stage (R0. 05) and the differentiation of tumor (RQ. 05), but have no relationship with sex and histology (P>0. 05); the positive expression of E-cadherin was related with age(K0.05) > TNM stage(K0. 005) and thedifferentiation of tumor(FKO. 05), but have no association with sex> histology^ tumor size and lymph node metastasis (/>0. 05); the positive express of p27 was only related with the differentiation of tumor (K0. 05), but have no association with age^ sex% histology^ tumor size> lymph node metastasis and TNM stage(P>0. 05).3. The expression of S100A4 inversely correlated with that of E-cadherin(K0. 05) in NSCLC, however, there was no correlation between the positive expression of S100A4 and p27(/5>0. 05). Conclusion: 1. It may have important clinic significance to judge the biological behavior and prognosis of NSCLC when combining to examine the expression of S100A4, E-cadherin and p27;2. The abnormal expression rates of S100A4 and p27 were very high in NSCLC, but there was no significant inverse correlation between them;3. S100A4 may independently play an important role in predicting of the clinicopathologic features of NSCLC; Moreover, it possibly may be a new target for therapy of lung cancer.