The Research On Association Between Helicobacter Infection And Primary Liver Carcinoma

Objective: To investigate the relationship between Helicobacter species and primary liver carcinoma(PLC) with molecular biology technique.Methods: 1.The liver samples resected during operation from 48 patients with PLC diagnosed by histopathology and 29 patients with other liver diseases as controls were studied, and the respective tumor tissues from 30 patients with gastric cancer ,20 patients with colon carcinoma and 20 patients with uterus myoma were also studied as controls. The 16SrRNA gene and function- associated gene cagA, vacA in Helicobacter species were examined by PCR, and the amplified products of 16SrRNA were identified by Southern hybridization and received with homology and sequence analysis. 2 .Helicobacter species in the specimens from the studied subjects were cultured with Columbia culture medium, and then were stained and identified by urease test if the culture results were positive. The 16SrRNA gene and function- associated gene cagA, vacA in cultured Helicobacter species were also examined. The study results were used to assess the correlation of liver tissue helicobacter infection with PLC. Results: l.The Helicobacter species were detected after culture in 5 of 48 liver carcinoma samples( 10.42%) and 7 of 30 gastric cancer samples (23.33%) respectively , and all helicobacter species were proved by gram staining, urease test , 16SrRNA gene detection , Southern hybridization and sequence analysis. 2. 29 of the 48 samples (60.4%) of PLC and 20 of the 30 samples (66.7%) of gastric cancer were positive for Helicobacter specific 16SrRNA gene, while none was positive in the other controls. 3. Function-associated gene cagA were found in 5 samples of PLC and 2 samples of gastric cancer, but the vacA gene was not found in all samples.Conclusions: Helicobacter infection may exist in the liver tissues of PLC patients with a high infection rate, and most of their genotypes are type â…¡.