Expression Of CD82 And VEGF In Small Hepatocellular Carcinoma And Its Association With Postoperative Recurrence And Metastasis

Purposes: To investigate the relationship between expression of tumor metastasis-suppressing gene KAI1/CD82 and VEGF in small hepatocellular carcinoma (SHCC) and its association with clinopathological parameters and postoperative recurrence and metastasis.Methods: Expression of CD82 and VEGF were determined by immunohistochemistry technique (S-P method) in 40 cases of SHCC tissue after being curative resection and 35 cases of its adjacent liver tissue and 5 cases of hepatic adenoma tissue and 5 cases of normal liver tissue from liver angioma. Combining the clinopathological parameters (include the serum level of AFP, the status of capsule and the degree of differentiation ) with the follow-up study were analyzed.Results: (1) The positive rate of CD82 protein in SHCC was 40% (16/40) , which was significantly lower than that in the normal liver tissue (100%, 5/5) and hepatic adenoma tissue (100%, 5/5) and adjacent liver tissue (100%, 35/35) (p<0.05); The positive rate of VEGF protein in SHCC was 85% (34/40), which was significantly higher than that in the normal liver tissue (40%, 2/5) and adjacent liver tissue (42.8%, 15/35) (p<0.05). (2) Spearman correlation analysis did not show the expression of CD82 was correlated to the expression of VEGF. The expression of CD82 and VEGF were not correlated to the status of capsule , the serum level of AFP and the degree of differentiation respectively. (3) The recurrence and metastasis rate in CD82 negative SHCC group was 54.2%, which was significantly higher than that of in CD82 positive SHCC group (18.8%) (p=0.026).Life Table survival analysis showed that 1,2,3-year cumulative disease-free survival rates in CD82 positive SHCC group were 94%,...