Expression And Clinical-pathological Significance Of HIF-1α, VEGF, PCNA And Bcl-2 In Breast Cancer

Objective To investigate the expression of HIF-1α, VEGF,PCNA and Bcl-2 and theirrelationship with clinical-pathological factors in breast cancer .Methods To detect the expression of HIF-1α, VEGF, PCNA and Bcl-2 in paraffinembedment specimens of 15 breast non-cancer tissues and 60 breast cancers by immunohistochemical SABC method respectively, and to analyze the relationship between the expression and some clinical-pathological factors such as the age of onset, menstrual status, tumor size, clinical TNM stage, histological grade, axillary lymph node metastasis, levels of estrogen receptor (ER) and progesterone receptor (PR). And to study the relationship between HIF-la, VEGF, PCNA and Bcl-2.Results (1) HIF-1α, VEGF, Bcl-2 mainly expressed in the cytoplasm and PCNAmainly expressed in the nucleus of tumor cells. (2) HIF-1α don't express in 15 breast non-cancer tissues, the positive rate of HIF-1α was 55% in 60 breast cancers, there was a significant differentce between them(P<0.05). The positive rate of VEGF was 30% and 71.67% in 15 breast non-cancer tissues and 60 breast cancers respectively, there was a significant differentce between them(P<0.05). In breast cancer, their expressions were positively correlated with axillary lymph node metastasis and histological grade (P<0.05), and were not correlated with other clinical-pathological factors. The positive rate of PCNA was 13.3% and 76.67% in 15 breast non-cancer tissues and 60 breast cancers respectively, there was a significant differentce between them(P<0.05), its expression was positively correlated with tumor size and histological grade, while was negatively correlated with estrogen receptor (ER) level, and was not correlated with other clinical-pathological factors. The positive rate of Bcl-2 was 100% and 61.67% in 15 breast non-cancer tissues and 60 breast cancers respectively, there was a significant differentce between them(P<0.05), its...