Efficacy Of Sustained Released Fluorouracil For Intrutumoral Chemotherapy Of Breast Cancer And The Molecule Mechanisms Of Inducing Apoptosis

Objective The purpose of this study is to investigate the efficacy of intrutumoral chemotherapy of sustained released Fluorouraci in treatment for local advanced breast cancer and molecular biology mechanisms through observing the difference and correlation of AI (Apoptosis index ) the expression of PCNA( Proliferating cell nuclear antigen) > MVD(microvessel density) the experession of P53 and Puma(P53 up-regulated modulator of apoptosis) between the intrutumoral chemotherapy group and the control group.Methods Inclusion criteria of patients in intratumoral chemotherapy group is staging of III and part of IIB (T3N0M0) diagnosed by biopsy pathology and the control group is selected by pairing of age the condition of menstruation the TNM staging lymphatic metaptosis with intratumoral chemotherapy group. The age difference must not exceed than five. The patients of intratumoral chemotherapy group is implantated with sustained released Fluorouraci 300-600mg intratumorally and after 7-10 day they will been operated. After operation the chemotherapy is given as routine. Observing the clinical effective and the adverse effect. Apoptosis index of tumor cells is assayed by TUNEL(TdT-mediated dUTP nick end labeling) and PCNA CD34 P53 and Puma is assayed by immunohistochemistry in specimen of breast cancer in two groups, observing the difference and correlation of Apoptosis index the expression of proliferating cell nuclear antigen micro vessel density the experession of P53 and Puma between two groups. results Intrutumoral chemotherapy of sustained released Fluorouracil is effective and...